-
Tytuł:
-
Potential Phosphorylation of Viral Nonstructural Protein 1 in Dengue Virus Infection.
-
Autorzy:
-
Dechtawewat T; Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Roytrakul S; Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 12120, Thailand.
Yingchutrakul Y; Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 12120, Thailand.
Charoenlappanit S; Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 12120, Thailand.
Siridechadilok B; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Limjindaporn T; Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Mangkang A; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Prommool T; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Puttikhunt C; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Songprakhon P; Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Kongmanas K; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Kaewjew N; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Avirutnan P; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Yenchitsomanus PT; Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Malasit P; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Noisakran S; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10700, Thailand.; Division of Dengue Hemorrhagic Fever Research, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
-
Źródło:
-
Viruses [Viruses] 2021 Jul 17; Vol. 13 (7). Date of Electronic Publication: 2021 Jul 17.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Basel, Switzerland : MDPI
-
MeSH Terms:
-
Dengue Virus/*chemistry
Dengue Virus/*pathogenicity
Viral Nonstructural Proteins/*metabolism
Animals ; Cell Line ; Chlorocebus aethiops ; Chromatography, Liquid ; Dengue/virology ; Dengue Virus/genetics ; Hep G2 Cells ; Humans ; Kinetics ; Phosphorylation ; Protein Binding ; Sequence Analysis, Protein ; Tandem Mass Spectrometry ; Vero Cells ; Viral Nonstructural Proteins/genetics ; Virus Replication
-
References:
-
PLoS Pathog. 2007 Nov;3(11):e183. (PMID: 18052531)
Virus Res. 2005 Sep;112(1-2):85-94. (PMID: 15878791)
Nature. 2013 Apr 25;496(7446):504-7. (PMID: 23563266)
Cell Host Microbe. 2009 Apr 23;5(4):365-75. (PMID: 19380115)
Proc Natl Acad Sci U S A. 2011 May 10;108(19):8003-8. (PMID: 21518917)
Bioessays. 2015 May;37(5):489-94. (PMID: 25761098)
Virol J. 2015 Feb 06;12:14. (PMID: 25890165)
Biochem Biophys Res Commun. 2007 Nov 3;362(4):1051-6. (PMID: 17825259)
J Immunol. 2016 Nov 15;197(10):4053-4065. (PMID: 27798151)
J Virol. 2015 Dec;89(23):11871-83. (PMID: 26378175)
FASEB J. 2000 Aug;14(11):1603-10. (PMID: 10928995)
J Biol Chem. 2011 Apr 1;286(13):11506-18. (PMID: 21177246)
J Gen Virol. 2008 Oct;89(Pt 10):2492-2500. (PMID: 18796718)
Clin Exp Immunol. 1979 Apr;36(1):46-53. (PMID: 380857)
J Exp Med. 2010 Apr 12;207(4):793-806. (PMID: 20308361)
J Infect Dis. 2020 Dec 15;:. (PMID: 33319249)
Electrophoresis. 1999 Dec;20(18):3551-67. (PMID: 10612281)
J Virol. 1997 Dec;71(12):9608-17. (PMID: 9371625)
Trends Pharmacol Sci. 2008 Aug;29(8):413-20. (PMID: 18606460)
Virology. 1996 Jun 1;220(1):232-40. (PMID: 8659120)
Sci Transl Med. 2015 Sep 9;7(304):304ra141. (PMID: 26355030)
Science. 2014 Feb 21;343(6173):881-5. (PMID: 24505133)
Virol J. 2013 Nov 18;10:339. (PMID: 24245822)
Virus Res. 1997 May;49(1):9-15. (PMID: 9178492)
BMC Infect Dis. 2019 Aug 28;19(1):750. (PMID: 31455279)
J Virol. 1998 Jul;72(7):6199-206. (PMID: 9621090)
J Infect Dis. 2006 Apr 15;193(8):1078-88. (PMID: 16544248)
J Virol. 2009 Sep;83(18):9195-205. (PMID: 19587048)
Curr Opin Microbiol. 2008 Aug;11(4):369-77. (PMID: 18644250)
Virology. 2010 Jan 5;396(1):30-6. (PMID: 19897220)
Viruses. 2015 Oct 12;7(10):5257-73. (PMID: 26473910)
J Cell Biol. 2007 Apr 9;177(1):127-37. (PMID: 17403928)
J Immunol. 2011 Jul 1;187(1):424-33. (PMID: 21642539)
J Cell Physiol. 2015 Feb;230(2):449-63. (PMID: 25078272)
PLoS One. 2018 Mar 16;13(3):e0194399. (PMID: 29547653)
J Virol. 2005 Sep;79(17):11403-11. (PMID: 16103191)
Biochim Biophys Acta. 2016 Sep;1864(9):1270-1280. (PMID: 27108190)
Front Immunol. 2020 Jun 18;11:1252. (PMID: 32655561)
J Virol Methods. 2007 Jun;142(1-2):67-80. (PMID: 17331594)
J Virol. 2011 Feb;85(3):1158-73. (PMID: 21084474)
J Biol Chem. 1995 Aug 11;270(32):19100-6. (PMID: 7642575)
PLoS Pathog. 2015 Nov 12;11(11):e1005277. (PMID: 26562291)
Cell Rep. 2018 Jan 30;22(5):1364. (PMID: 29386121)
J Gen Virol. 2018 Oct;99(10):1391-1406. (PMID: 30102148)
Trends Biochem Sci. 2000 Dec;25(12):596-601. (PMID: 11116185)
Arch Virol. 2011 Jul;156(7):1275-9. (PMID: 21424730)
J Immunol. 1970 Dec;105(6):1565-8. (PMID: 5483833)
Virology. 2015 Oct;484:113-126. (PMID: 26092250)
J Gen Virol. 2020 May;101(5):484-496. (PMID: 32141809)
Thromb Haemost. 2008 May;99(5):936-43. (PMID: 18449425)
PLoS One. 2013;8(3):e57514. (PMID: 23516407)
J Virol. 2013 Dec;87(23):12667-74. (PMID: 24049164)
Virology. 2011 May 10;413(2):253-64. (PMID: 21429549)
N Engl J Med. 2018 Jul 26;379(4):327-340. (PMID: 29897841)
Nat Rev Mol Cell Biol. 2007 Jul;8(7):530-41. (PMID: 17585314)
J Infect Dis. 2002 Oct 15;186(8):1165-8. (PMID: 12355369)
PLoS Negl Trop Dis. 2020 Nov 20;14(11):e0008835. (PMID: 33216752)
Curr Opin Virol. 2020 Aug;43:71-78. (PMID: 33086187)
Virology. 1989 Jul;171(1):302-5. (PMID: 2525840)
Front Genet. 2014 Aug 07;5:270. (PMID: 25147561)
J Clin Microbiol. 2000 Mar;38(3):1053-7. (PMID: 10698995)
PLoS Pathog. 2019 May 9;15(5):e1007736. (PMID: 31071189)
Rev Med Virol. 2012 May;22(3):166-81. (PMID: 22113983)
Sci Transl Med. 2015 Sep 9;7(304):304ra142. (PMID: 26355031)
Virology. 1988 Jan;162(1):187-96. (PMID: 2827377)
Virology. 2016 Sep;496:227-236. (PMID: 27348054)
J Infect Dis. 2009 Oct 15;200(8):1261-70. (PMID: 19754307)
J Virol. 2009 Jun;83(11):5408-18. (PMID: 19279106)
Am J Trop Med Hyg. 1985 Jan;34(1):162-9. (PMID: 2578750)
Am J Trop Med Hyg. 1982 Jul;31(4):830-6. (PMID: 6285749)
J Gen Virol. 2012 Apr;93(Pt 4):771-779. (PMID: 22238236)
Mol Cell Proteomics. 2011 Dec;10(12):M111.012187. (PMID: 21911577)
PeerJ. 2020 Sep 25;8:e9988. (PMID: 33033661)
J Virol. 1997 Sep;71(9):6650-61. (PMID: 9261387)
J Clin Microbiol. 2002 Feb;40(2):376-81. (PMID: 11825945)
Viruses. 2017 Apr 24;9(4):. (PMID: 28441781)
J Virol. 1999 Jul;73(7):6104-10. (PMID: 10364366)
J Virol Methods. 2003 Apr;109(1):55-61. (PMID: 12668268)
PLoS Pathog. 2015 Jul 30;11(7):e1005053. (PMID: 26226614)
-
Contributed Indexing:
-
Keywords: LC-MS/MS; NS1; dengue virus; phosphorylation; virus production
-
Substance Nomenclature:
-
0 (NS1 protein, Dengue virus type 2)
0 (Viral Nonstructural Proteins)
-
Entry Date(s):
-
Date Created: 20210810 Date Completed: 20211118 Latest Revision: 20211118
-
Update Code:
-
20240105
-
PubMed Central ID:
-
PMC8310366
-
DOI:
-
10.3390/v13071393
-
PMID:
-
34372598
-
Dengue virus (DENV) infection causes a spectrum of dengue diseases that have unclear underlying mechanisms. Nonstructural protein 1 (NS1) is a multifunctional protein of DENV that is involved in DENV infection and dengue pathogenesis. This study investigated the potential post-translational modification of DENV NS1 by phosphorylation following DENV infection. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), 24 potential phosphorylation sites were identified in both cell-associated and extracellular NS1 proteins from three different cell lines infected with DENV. Cell-free kinase assays also demonstrated kinase activity in purified preparations of DENV NS1 proteins. Further studies were conducted to determine the roles of specific phosphorylation sites on NS1 proteins by site-directed mutagenesis with alanine substitution. The T27A and Y32A mutations had a deleterious effect on DENV infectivity. The T29A, T230A, and S233A mutations significantly decreased the production of infectious DENV but did not affect relative levels of intracellular DENV NS1 expression or NS1 secretion. Only the T230A mutation led to a significant reduction of detectable DENV NS1 dimers in virus-infected cells; however, none of the mutations interfered with DENV NS1 oligomeric formation. These findings highlight the importance of DENV NS1 phosphorylation that may pave the way for future target-specific antiviral drug design.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.
