A simple and fast spectroscopy-based technique for Covid-19 diagnosis.

Tytuł:: A simple and fast spectroscopy-based technique for Covid-19 diagnosis.
Autorzy:: Kitane DL; Operations Research Center, MIT, Muckley Bldg, 1 Amherst St, Cambridge, MA, 02142, USA.
Loukman S; Anoual Laboratory, Boulevard d'Alexandrie, 20360, Casablanca, Morocco.
Marchoudi N; Anoual Laboratory, Boulevard d'Alexandrie, 20360, Casablanca, Morocco.
Fernandez-Galiana A; LIGO Laboratory, MIT, 185 Albany Street, Cambridge, MA, 02139, USA.
El Ansari FZ; Anoual Laboratory, Boulevard d'Alexandrie, 20360, Casablanca, Morocco.
Jouali F; Anoual Laboratory, Boulevard d'Alexandrie, 20360, Casablanca, Morocco.
Badir J; Centre for Applied Molecular Technologies (CTMA), Université Catholique de Louvain, Louvain, Belgium.
Gala JL; Centre for Applied Molecular Technologies (CTMA), Université Catholique de Louvain, Louvain, Belgium.
Bertsimas D; Operations Research Center, MIT, Muckley Bldg, 1 Amherst St, Cambridge, MA, 02142, USA.
Azami N; Photonics Labs, INPT, Madinat Al Irfane, Rabat, Morocco.
Lakbita O; Chemical and Biochemical Sciences Department (CBS), Mohammed VI Polytechnic University, UM6P. Lot 660, Hay Moulay Rachid, 43150, Benguerir, Morocco.
Moudam O; Chemical and Biochemical Sciences Department (CBS), Mohammed VI Polytechnic University, UM6P. Lot 660, Hay Moulay Rachid, 43150, Benguerir, Morocco.
Benhida R; Chemical and Biochemical Sciences Department (CBS), Mohammed VI Polytechnic University, UM6P. Lot 660, Hay Moulay Rachid, 43150, Benguerir, Morocco. .; Nice Institute of Chemistry, University Côte d'Azur, Nice, France. .
Fekkak J; Anoual Laboratory, Boulevard d'Alexandrie, 20360, Casablanca, Morocco.
Źródło:: Scientific reports [Sci Rep] 2021 Aug 18; Vol. 11 (1), pp. 16740. Date of Electronic Publication: 2021 Aug 18.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Spectroscopy, Fourier Transform Infrared*
COVID-19 Testing/*methods
RNA, Viral/*analysis
Humans ; RNA, Viral/chemistry ; RNA, Viral/isolation & purification ; Time Factors
References:: Clin Chim Acta. 2020 Jun;505:172-175. (PMID: 32156607)
Biochem Biophys Res Commun. 2004 Nov 12;324(2):634-9. (PMID: 15474474)
Front Immunol. 2020 Oct 14;11:585354. (PMID: 33163000)
Nature. 2020 May;581(7809):465-469. (PMID: 32235945)
Biosens Bioelectron. 2021 Apr 15;178:113012. (PMID: 33497879)
Nat Biotechnol. 2020 Oct;38(10):1168-1173. (PMID: 32733106)
N Engl J Med. 2020 Mar 19;382(12):1177-1179. (PMID: 32074444)
N Engl J Med. 2020 Dec 17;383(25):2427-2438. (PMID: 32991794)
Clin Immunol. 2020 Jun;215:108427. (PMID: 32325252)
Science. 2020 May 29;368(6494):945-946. (PMID: 32385100)
Lancet. 2020 Mar 14;395(10227):871-877. (PMID: 32087820)
ACS Nano. 2020 Apr 28;14(4):3822-3835. (PMID: 32223179)
Anal Chem. 2021 Feb 9;93(5):2950-2958. (PMID: 33481583)
J Infect. 2013 May;66(5):391-400. (PMID: 23103289)
PLoS Biol. 2020 Dec 15;18(12):e3001030. (PMID: 33320856)
Methods Mol Biol. 2020;2113:119-133. (PMID: 32006312)
Science. 2021 Feb 19;371(6531):850-854. (PMID: 33495308)
Cell Discov. 2020 May 19;6:34. (PMID: 32435508)
J Public Health Policy. 2018 Aug;39(3):268-282. (PMID: 30013135)
N Engl J Med. 2014 Apr 3;370(14):1335-42. (PMID: 24693893)
Lancet. 2021 Jan 9;397(10269):99-111. (PMID: 33306989)
J Clin Microbiol. 2020 Aug 24;58(9):. (PMID: 32651238)
Rev Med Virol. 2020 May;30(3):e2106. (PMID: 32302058)
J Clin Virol. 2020 Jul;128:104416. (PMID: 32388470)
Talanta. 2000 Oct 2;53(1):233-46. (PMID: 18968108)
Chem Soc Rev. 2016 Apr 7;45(7):1980-98. (PMID: 26403652)
BMC Infect Dis. 2019 Feb 6;19(1):117. (PMID: 30727970)
Lancet. 2020 Mar 28;395(10229):1054-1062. (PMID: 32171076)
Nat Biotechnol. 2020 Jul;38(7):870-874. (PMID: 32300245)
Clin Chem. 2020 Jul 1;66(7):977-979. (PMID: 32282874)
Trends Analyt Chem. 2017 Dec;97:244-256. (PMID: 32287542)
PLoS One. 2015 Nov 16;10(11):e0143164. (PMID: 26569120)
Emerg Infect Dis. 2006 Jan;12(1):15-22. (PMID: 16494711)
Nat Rev Drug Discov. 2020 May;19(5):305-306. (PMID: 32273591)
Emerg Microbes Infect. 2020 Dec;9(1):1300-1308. (PMID: 32458760)
J Intern Med. 2020 Aug;288(2):192-206. (PMID: 32348588)
Vaccine. 2017 Aug 16;35(35 Pt A):4470-4474. (PMID: 28633891)
Emerg Microbes Infect. 2020 Dec;9(1):940-948. (PMID: 32357808)
Sci Adv. 2018 May 23;4(5):eaat0496. (PMID: 29806030)
Nat Biomed Eng. 2020 Dec;4(12):1159-1167. (PMID: 33273713)
Adv Drug Deliv Rev. 2021 May;172:314-338. (PMID: 33482248)
Expert Rev Mol Diagn. 2020 Jul;20(7):647-649. (PMID: 32378969)
Proc Natl Acad Sci U S A. 2020 Sep 15;117(37):22727-22735. (PMID: 32868442)
Nat Rev Dis Primers. 2017 Mar 02;3:17006. (PMID: 28252637)
Substance Nomenclature:: 0 (RNA, Viral)
Entry Date(s):: Date Created: 20210819 Date Completed: 20210825 Latest Revision: 20220105
Update Code:: 20240104
PubMed Central ID:: PMC8373901
DOI:: 10.1038/s41598-021-95568-5
PMID:: 34408169
: Czasopismo naukowe

Pełny tekst

The coronavirus pandemic, which appeared in Wuhan, China, in December 2019, rapidly spread all over the world in only a few weeks. Faster testing techniques requiring less resources are key in managing the pandemic, either to enable larger scale testing or even just provide developing countries with limited resources, particularly in Africa, means to perform tests to manage the crisis. Here, we report an unprecedented, rapid, reagent-free and easy-to-use screening spectroscopic method for the detection of SARS-CoV-2 on RNA extracts. This method, validated on clinical samples collected from 280 patients with quantitative predictive scores on both positive and negative samples, is based on a multivariate analysis of FTIR spectra of RNA extracts. This technique, in agreement with RT-PCR, achieves 97.8% accuracy, 97% sensitivity and 98.3% specificity while reducing the testing time post RNA extraction from hours to minutes. Furthermore, this technique can be used in several laboratories with limited resources.
(© 2021. The Author(s).)

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