Regulation of the Tpo, Tg, Duox2, Pds, and Mct8 genes involved in the synthesis of thyroid hormones after subchronic exposure to sodium nitrate in female Wistar rats.

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Abstrakt

Tytuł:: Regulation of the Tpo, Tg, Duox2, Pds, and Mct8 genes involved in the synthesis of thyroid hormones after subchronic exposure to sodium nitrate in female Wistar rats.
Autorzy:: Ríos-Sánchez E; Laboratorio de Biología Celular y Molecular, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, Mexico.
González-Zamora A; Laboratorio de Biología Evolutiva. Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Durango, Mexico.
Gonsebatt Bonaparte ME; Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
Meza Mata E; Departamento de Patología, Unidad Médica de Alta Especialidad #71. Instituto Mexicano del Seguro Social, Torreón, Mexico.
González-Delgado MF; Laboratorio de Biología Celular y Molecular, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, Mexico.
Zámago Amaro A; Laboratorio de Biología Celular y Molecular, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, Mexico.
Pérez-Morales R; Laboratorio de Biología Celular y Molecular, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, Mexico.
Źródło:: Environmental toxicology [Environ Toxicol] 2021 Dec; Vol. 36 (12), pp. 2380-2391. Date of Electronic Publication: 2021 Aug 18.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: New York, NY : John Wiley & Sons, c1999-
MeSH Terms:: Nitrates*
Thyroid Hormones*
Animals ; Dual Oxidases ; Female ; Rats ; Rats, Wistar
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Grant Information:: 467347 Consejo Nacional de Ciencia y Tecnología; Sistema Nacional de Investigadores of CONACYT; Doctorado en Ciencias Biomédicas of the UJED
Contributed Indexing:: Keywords: disruptor hormones; gene expression; liver; nitrate; thyroid
Substance Nomenclature:: 0 (Nitrates)
0 (Thyroid Hormones)
8M4L3H2ZVZ (sodium nitrate)
EC 1.11.1.- (Dual Oxidases)
Entry Date(s):: Date Created: 20210819 Date Completed: 20211104 Latest Revision: 20211104
Update Code:: 20240104
DOI:: 10.1002/tox.23351
PMID:: 34409734
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Nitrates are natural compounds present in soil and water; however, the intense use of fertilizers has increased their presence in groundwater with deleterious effects on human health. There is evidence of nitrates acting as endocrine disruptors; however, the underlying molecular mechanisms have not been fully described. Here, we investigated the effect of subchronic exposure to different concentrations of sodium nitrate in female Wistar rats, evaluating thyroid hormonal parameters, such as Nis transporter (Na + /I - symporter, Slc5a5) and Tsh-R receptor protein expression, as well as transcription of the Tpo (thyroperoxidase), Tg (tiroglobulin), Duox2 (dual oxidase 2), Pds (pendrin), and Mct8 (Mct8 transporter, Slc16a2) genes. Hematological and histochemical changes in the liver and thyroid were also explored. Significant differences were found in platelet and leukocyte counts; although a significant increase in the weight of the thyroid gland was observed, no differences were found in the levels of the hormones Tsh, T3, and T4, but a modulation of the mRNA expression of the Tg, Tpo, Duox2, Mct8, and Pds genes was observed. Morphological changes were also found in liver and thyroid tissue according to the exposure doses. In conclusion, subchronic exposure to sodium nitrate induces leukocytosis consistent with an inflammatory response and upregulation of Sod2 in the liver and increases the expression of genes involved in the synthesis of thyroid hormones, keeping thyroid hormone levels stable. Histological changes in the thyroid gland suggest a goitrogenic effect.
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