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Tytuł pozycji:

Implementations of temperature gradients in temperature-responsive liquid chromatography.

Tytuł:
Implementations of temperature gradients in temperature-responsive liquid chromatography.
Autorzy:
Baert M; Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4bis, Ghent B-9000, Belgium.
Wicht K; Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4bis, Ghent B-9000, Belgium.
Moussa A; Department of Chemical Engineering, Vrije Universiteit Brussel, Pleinlaan 2, Brussel B-1050, Belgium.
Desmet G; Department of Chemical Engineering, Vrije Universiteit Brussel, Pleinlaan 2, Brussel B-1050, Belgium.
Broeckhoven K; Department of Chemical Engineering, Vrije Universiteit Brussel, Pleinlaan 2, Brussel B-1050, Belgium.
Lynen F; Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4bis, Ghent B-9000, Belgium. Electronic address: .
Źródło:
Journal of chromatography. A [J Chromatogr A] 2021 Sep 27; Vol. 1654, pp. 462425. Date of Electronic Publication: 2021 Jul 22.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Amsterdam ; New York : Elsevier, 1993-
MeSH Terms:
Chemistry Techniques, Analytical*/methods
Chromatography, High Pressure Liquid*
Temperature*
Solutions ; Solvents
Contributed Indexing:
Keywords: Axial temperature gradients; Peak refocusing; Radial temperature gradients; Temperature gradients; Temperature-responsive liquid chromatography; Temperature-responsive polymer
Substance Nomenclature:
0 (Solutions)
0 (Solvents)
Entry Date(s):
Date Created: 20210823 Date Completed: 20211013 Latest Revision: 20211013
Update Code:
20240105
DOI:
10.1016/j.chroma.2021.462425
PMID:
34425285
Czasopismo naukowe
Temperature Responsive Liquid Chromatography (TRLC) offers an alternative and environmentally friendly way to perform reversed-phase like separations. Its use of temperature responsive polymers to control retention based on column temperature, instead of the fraction of organic modifier in the mobile phase mobile, eliminates the need for solvent composition gradients and allows, for example, for purely aqueous separations. In principle this temperature induced retention should allow for gradient elutions to be performed using downward temperature gradients to control retention and refocus the analyte peaks. Yet, the unavailability of dedicated commercial temperature controlling systems allowing suitable temperature control in TRLC limits implementations thereof often to isothermal or step gradient applications. In this work we study the potential of 1) a simple yet programmable water bath and of 2) a modified HPLC system allowing column temperature programming through controlled mixing of a warm and cold mobile phase streams. The performance of both systems was evaluated under both isocratic and gradient applications, resulting in a more thorough understanding of the influence of temperature gradients in TRLC. This knowledge is then applied to a sample of phenolic solutes, illustrating that, although both systems have some flaws, both are able to impose temperature gradients in TRLC resulting in significantly reduced retention and enhanced refocusing of the analyte peak.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021. Published by Elsevier B.V.)

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