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Tytuł:
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Adenosine triphosphate (ATP) and zinc(II) ions responsive pyrene based turn-on fluorescent probe and its application in live cell imaging.
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Autorzy:
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Zhang X; College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China.
Liu J; Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450016, Henan, China.
Wang J; Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450016, Henan, China.
Han L; Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450016, Henan, China.
Ma S; Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450016, Henan, China.
Zhao M; College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China. Electronic address: .
Xi G; Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450016, Henan, China. Electronic address: .
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Źródło:
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Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2021 Oct; Vol. 223, pp. 112279. Date of Electronic Publication: 2021 Aug 04.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Lausanne : Elsevier Sequoia, 1987-
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MeSH Terms:
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Adenosine Triphosphate/*analysis
Fluorescent Dyes/*chemistry
Pyrenes/*chemistry
Zinc/*chemistry
Adenosine Triphosphate/chemistry ; Density Functional Theory ; Fluorescent Dyes/analysis ; HeLa Cells ; Humans ; Ions/chemistry ; Limit of Detection ; Magnetic Resonance Spectroscopy ; Microscopy, Fluorescence ; Quantum Theory ; Schiff Bases/chemistry ; Zinc/metabolism
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Contributed Indexing:
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Keywords: ATP sensor; Chemosensor; Imaging; Pyrene; Zinc
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Substance Nomenclature:
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0 (Fluorescent Dyes)
0 (Ions)
0 (Pyrenes)
0 (Schiff Bases)
8L70Q75FXE (Adenosine Triphosphate)
J41CSQ7QDS (Zinc)
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Entry Date(s):
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Date Created: 20210823 Date Completed: 20211006 Latest Revision: 20211006
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Update Code:
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20240105
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DOI:
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10.1016/j.jphotobiol.2021.112279
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PMID:
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34425416
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A novel highly selective and sensitive turn-on fluorescent chemosensor PCE to recognize Zn 2+ has been developed. The sensor PCE displays a remarkable fluorescent enhancement at 456 nm (λ ex = 340 nm) with Zn 2+ without the interference of other biologically important relevant metal ions in aqueous acetonitrile solution. Job's plot and mass spectral studies divulge such the interaction of PCE by Zn 2+ was 1:1 binding stoichiometry. The association constant and detection limit of PCE to recognize Zn 2+ was found to be 0.948 × 10 4 M -1 and 4.82 × 10 -7 M respectively. The nature of turn-on fluorescence sensor was supported by TD-DFT calculations. And the synthesized probe PCE was able to image intracellular Zn 2+ in living cells using confocal imaging techniques. PCE-Zn ensemble showed the remarkable fluorescence enhancement with ATP selectively among other biologically important phosphates. 31 P NMR experiments suggesting that the triphosphates unit of ATP is intact with the PCEZn. PCE-Zn ensemble can be utilized for monitoring ATP in live cells.
(Copyright © 2021. Published by Elsevier B.V.)
