Pumilio2 Promotes Growth of Mature Neurons.

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Abstrakt

Tytuł:: Pumilio2 Promotes Growth of Mature Neurons.
Autorzy:: Schieweck R; Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, Germany.
Schöneweiss EC; Zentrum für Medizinische Biotechnologie (ZMB), University of Duisburg-Essen, 41541 Duisburg, Germany.
Harner M; Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, Germany.
Rieger D; Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, Germany.
Illig C; Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, Germany.
Saccà B; Zentrum für Medizinische Biotechnologie (ZMB), University of Duisburg-Essen, 41541 Duisburg, Germany.
Popper B; Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, Germany.; Biomedical Center (BMC), Core Facility Animal Models, Ludwig-Maximilians-University, 82152 München, Germany.
Kiebler MA; Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, Germany.
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2021 Aug 20; Vol. 22 (16). Date of Electronic Publication: 2021 Aug 20.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:: Eukaryotic Initiation Factor-4E/*metabolism
Neurons/*metabolism
RNA-Binding Proteins/*metabolism
Animals ; Eukaryotic Initiation Factor-4E/genetics ; Female ; Fibroblast Growth Factor 2/metabolism ; Gene Expression/genetics ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Atomic Force/methods ; Neurogenesis/physiology ; Protein Binding/physiology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA-Binding Proteins/genetics ; Transcriptome/genetics
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Grant Information:: to RS Boehringer Ingelheim Fonds; FOR2333 to MAK Deutsche Forschungsgemeinschaft; SPP1738 to MAK Deutsche Forschungsgemeinschaft; 02/14 to BP Friedrich-Baur-Stiftung
Contributed Indexing:: Keywords: Pumilio2; atomic force microscopy; eIF4E; neuronal growth
Substance Nomenclature:: 0 (Eukaryotic Initiation Factor-4E)
0 (Pum2 protein, mouse)
0 (RNA, Messenger)
0 (RNA-Binding Proteins)
0 (eIF4E protein, mouse)
103107-01-3 (Fibroblast Growth Factor 2)
Entry Date(s):: Date Created: 20210827 Date Completed: 20210928 Latest Revision: 20210928
Update Code:: 20240105
PubMed Central ID:: PMC8396670
DOI:: 10.3390/ijms22168998
PMID:: 34445704
: Czasopismo naukowe

Pełny tekst

RNA-binding proteins (RBPs) are essential regulators controlling both the cellular transcriptome and translatome. These processes enable cellular plasticity, an important prerequisite for growth. Cellular growth is a complex, tightly controlled process. Using cancer cells as model, we looked for RBPs displaying strong expression in published transcriptome datasets. Interestingly, we found the Pumilio (Pum) protein family to be highly expressed in all these cells. Moreover, we observed that Pum2 is regulated by basic fibroblast growth factor (bFGF). bFGF selectively enhances protein levels of Pum2 and the eukaryotic initiation factor 4E (eIF4E). Exploiting atomic force microscopy and in vitro pulldown assays, we show that Pum2 selects for eIF4E mRNA binding. Loss of Pum2 reduces eIF4E translation. Accordingly, depletion of Pum2 led to decreased soma size and dendritic branching of mature neurons, which was accompanied by a reduction in essential growth factors. In conclusion, we identify Pum2 as an important growth factor for mature neurons. Consequently, it is tempting to speculate that Pum2 may promote cancer growth.

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