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Tytuł:
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Translatome analysis reveals the regulatory role of betaine in high fat diet (HFD)-induced hepatic steatosis.
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Autorzy:
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Huang T; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Yu J; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Luo Z; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Yu L; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Liu S; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Wang P; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Jia M; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Wu T; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Miao W; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Zhou L; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Song Z; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Zhang H; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China.
Li Y; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, 530004, PR China. Electronic address: .
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Źródło:
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Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Oct 20; Vol. 575, pp. 20-27. Date of Electronic Publication: 2021 Aug 25.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
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MeSH Terms:
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Betaine/*pharmacology
Diet, High-Fat/*adverse effects
Non-alcoholic Fatty Liver Disease/*metabolism
Animals ; Disease Models, Animal ; Gene Expression Profiling/methods ; Lipid Metabolism ; Lipotropic Agents/pharmacology ; Male ; Mice ; Mice, Inbred C57BL ; Non-alcoholic Fatty Liver Disease/pathology ; Non-alcoholic Fatty Liver Disease/prevention & control ; Protein Biosynthesis ; Random Allocation ; Sequence Analysis, RNA/methods ; Transcriptome
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Contributed Indexing:
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Keywords: Betaine; NAFLD; RNC-Seq; Translation ratio; Translatome
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Substance Nomenclature:
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0 (Lipotropic Agents)
3SCV180C9W (Betaine)
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Entry Date(s):
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Date Created: 20210828 Date Completed: 20211221 Latest Revision: 20211221
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Update Code:
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20240105
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DOI:
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10.1016/j.bbrc.2021.08.058
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PMID:
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34454176
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Non-alcoholic fatty liver disease (NAFLD) is a common disease with a multitude of complications. Increasing evidence shows that the dietary supplement with betaine, a natural chemical molecule, can effectively reduce the fat accumulation in the liver. Translational regulation is considered to play a vital role in gene expression, but whether betaine functions through the regulation of gene translational level is still unclear. To this end, RNC-seq (mRNAs bound to ribosome-nascent chain complex sequencing) and RNA-seq co-analyses were performed to identify betaine target genes by using the liver samples from high-fat diet adding betaine treated and high-fat diet treated mice. The results showed that betaine does play a lipid-lowering role by regulating the expression of gene translation levels; some NAFLD- and lipid metabolism-associated genes were differentially expressed at translational level, for example. And the translation ratio (TR) of gene significantly increased after betaine treatment. Finally, we identified a novel function gene, Gpc1, which may mediate the lipid-lowering effect of betaine in the liver. To sum up, this study depicted the molecular portrait of mice liver with or without betaine treatment from the angel of translatome and transcriptome, giving insights into the molecular mechanism of betaine-mediated lipid-lowering effect and also providing new clues for understanding and prevention of NAFLD.
Competing Interests: Declaration of competing interest Authors declare that there is no conflict of interest or any competing financial interest.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
