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Tytuł:
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Analysing the genomes of two tick-borne encephalitis viruses isolated in Hungary in 1952 and 2019.
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Autorzy:
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Egyed L; Veterinary Medical Research Institute, ELKH, Hungária krt. 21, Budapest 1143, Hungary. Electronic address: .
Biksi I; SCG Diagnosztika Ltd., Délegyháza, Hungary.
Varga T; SCG Diagnosztika Ltd., Délegyháza, Hungary.
Zöldi V; Department of Pest control, National Centre for Epidemiology, Budapest, Hungary.
Dán Á; Danam.Vet. Molbiol, Kőszeg, Hungary.
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Źródło:
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Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2021 Nov; Vol. 12 (6), pp. 101806. Date of Electronic Publication: 2021 Aug 13.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier, 2010-
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MeSH Terms:
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Genome, Viral*
Encephalitis Viruses, Tick-Borne/*genetics
Encephalitis, Tick-Borne/*virology
Encephalitis Viruses, Tick-Borne/classification ; Hungary ; Phylogeny ; Seasons ; Time Factors
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Contributed Indexing:
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Keywords: Tick borne encephalitis virus; Isolations; Sequencing; Analyzing of isolates; Migrating birds; Laboratory strain
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Entry Date(s):
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Date Created: 20210829 Date Completed: 20211109 Latest Revision: 20211109
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Update Code:
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20240105
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DOI:
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10.1016/j.ttbdis.2021.101806
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PMID:
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34455141
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The genomes of two Tick-borne encephalitis virus (TBEV) strains were fully sequenced and compared to those of known Hungarian strains. One was a laboratory strain (KEM-1) isolated in 1952, which had gone through hundreds of passages both on Vero cell cultures and in laboratory mice, while the other was a recent isolate (2019) from questing female ticks. The laboratory strain formed a monophyletic group with the already published 4 Hungarian strains on the evolutionary tree, located relatively close to Finnish (Kumlinge) and Russian (Absettarov) strains. This KEM-1 strain was phylogenetically distantly related both to the geographically close reference strain Neudörfl and the chronologically close Czech isolates from 1953. The 2019 isolate, KEM-195 was related to TBEV isolates from Southern Slovakia and Styria, and had the longest (328 nucleotides) deletion in its 3'-non-coding region among published sequences of strains of European subtype. Our results show that decades of laboratory passage have not altered the viral genome too much and that at least two distinct branches of TBEV strains circulate in Hungary.
(Copyright © 2021. Published by Elsevier GmbH.)
