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Tytuł:
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Comparative transcriptome analysis reveals differential gene expression in sterile and fertile rubber tree varieties during flower bud differentiation.
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Autorzy:
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Li F; College of Tropical Crops, Hainan University, Hainan, 570228, China.
Wang Y; College of Tropical Crops, Hainan University, Hainan, 570228, China.
Gao H; College of Tropical Crops, Hainan University, Hainan, 570228, China.
Zhang X; Rubber Research Institute, Chinese Academy of Topical Agricultural Sciences, State Center for Rubber Breeding, Danzhou, Hainan, 571737, China.
Zhuang N; College of Tropical Crops, Hainan University, Hainan, 570228, China. Electronic address: .
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Źródło:
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Journal of plant physiology [J Plant Physiol] 2021 Oct; Vol. 265, pp. 153506. Date of Electronic Publication: 2021 Aug 30.
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Typ publikacji:
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Comparative Study; Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Jena, Germany : Urban & Fischer
Original Publication: Stuttgart ; New York : G. Fischer, c1984-
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MeSH Terms:
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Gene Expression Profiling*
Fertility/*genetics
Flowers/*genetics
Flowers/*growth & development
Hevea/*genetics
Plant Infertility/*genetics
Gene Expression Regulation, Plant ; Genes, Plant ; Genetic Variation ; Genotype
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Contributed Indexing:
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Keywords: Differentially expressed genes; Expression pattern; Male sterility; Rubber tree; Transcriptome
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Entry Date(s):
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Date Created: 20210907 Date Completed: 20211005 Latest Revision: 20211005
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Update Code:
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20240105
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DOI:
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10.1016/j.jplph.2021.153506
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PMID:
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34492526
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Plant male sterility (MS) is an important agronomic trait that provides an efficient tool for hybridization and heterosis utilization of crops. Based on phenotypic and cytological observations, our study performed a multi-comparison transcriptome analysis strategy on multiple sterile and fertile rubber tree varieties using RNA-seq. Compared with the male-fertile varieties, a total of 1590 differentially expressed genes (DEGs) were detected in male-sterile varieties, including 970 up-regulated and 620 down-regulated transcripts in sterile varieties. Key DEGs were further assessed focusing on anther development, microsporogenesis and plant hormone metabolism. Twenty DEGs were selected randomly to validate transcriptome data using quantitative real-time PCR (qRT-PCR). Eleven key genes were subjected to expression pattern analysis using qRT-PCR and fluorescence in situ hybridization. Among them, nine genes, i.e., A6, GAI1, ACA7, TKPR1, CYP704B1, XTH26, MS1, MS35 and MYB33, that regulate callose metabolism, pollen wall formation, tapetum and microspores development were identified as candidate male-sterile genes. These findings provide insights into the molecular mechanism of male sterility in rubber tree.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
