Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Identification and expression analysis of miRNAs in germination and seedling growth of Tibetan hulless barley.

Tytuł:
Identification and expression analysis of miRNAs in germination and seedling growth of Tibetan hulless barley.
Autorzy:
Dou X; College of Agricultural Science and Technology, Tibet Vocational Technical College, Lhasa 850030, China. Electronic address: .
Zhou Z; College of Agricultural Science and Technology, Tibet Vocational Technical College, Lhasa 850030, China.
Zhao L; College of Agricultural Science and Technology, Tibet Vocational Technical College, Lhasa 850030, China.
Źródło:
Genomics [Genomics] 2021 Nov; Vol. 113 (6), pp. 3735-3749. Date of Electronic Publication: 2021 Sep 10.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: San Diego : Academic Press, [c1987-
MeSH Terms:
Hordeum*/metabolism
MicroRNAs*/genetics
MicroRNAs*/metabolism
Gene Expression Regulation, Plant ; Germination/genetics ; Seedlings/genetics ; Tibet
Contributed Indexing:
Keywords: Germination; Metabolism; MicroRNA; Seedling growth; Tibetan hulless barley (Hordeum vulgare L. var. nudum)
Substance Nomenclature:
0 (MicroRNAs)
Entry Date(s):
Date Created: 20210913 Date Completed: 20220331 Latest Revision: 20220401
Update Code:
20240105
DOI:
10.1016/j.ygeno.2021.08.019
PMID:
34517091
Czasopismo naukowe
Germination and seedling growth are crucial for plant development and agricultural production. While, the regulatory mechanisms during these processes in Tibetan hulless barley (Hordeum vulgare L. var. nudum) are not well understood. Given the regulatory roles of microRNAs (miRNAs) in crop plants and the irreplaceability of barley in the highland area of China, we herein presented a genome-wide survey of miRNAs to reveal a potential regulatory network in the early developmental stages of two Tibetan hulless barleys, from which a total of 156 miRNAs was identified including 35 known and 121 novel ones. Six of the identified novel miRNAs were further experimentally validated. According to the evolutionary analysis, miR156, miR166, miR168, and miR171 were conserved across Tibetan hulless barleys and eight other seed plants. Expression profiles of ten known miRNAs showed that they were involved in phytohormone signaling, carbohydrate and lipid metabolism, as well as juvenile-adult transition during barley development. Moreover, a total of 1280 genes targeted by 101 miRNAs were predicted from both barley libraries. Three genes (PLN03212, MATE eukaryotic, and GRAS) were validated via the RNA ligase-mediated 5'-rapid amplification of cDNA ends (RLM-5' RACE) to be the targets of hvu-miR159a, hvu-miR166a, and hvu-miR171-3p, respectively. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of putative targets, the most abundant pathways were related to "metabolism". These results revealed that miRNA-target pairs participating in the regulation of multigene expression and the embryonic development of Tibetan hulless barleys were controlled by complex mechanisms involving the concordant expression of different miRNAs and feedback loops among miRNAs as well as their targets. The study provides insight into the regulatory network of barley miRNAs for better understanding of miRNA functions during germination and seedling growth.
(Copyright © 2021 Elsevier Inc. All rights reserved.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies