Intravenous injection of mesenchymal stem cell spheroids improves the pulmonary delivery and prolongs in vivo survival.

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Tytuł:: Intravenous injection of mesenchymal stem cell spheroids improves the pulmonary delivery and prolongs in vivo survival.
Autorzy:: Shimazawa Y; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Kusamori K; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Tsujimura M; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Shimomura A; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Takasaki R; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Takayama Y; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Shimizu K; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, Japan.
Konishi S; Department of Mechanical Engineering, Graduate School of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga, Japan.
Nishikawa M; Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Źródło:: Biotechnology journal [Biotechnol J] 2022 Jan; Vol. 17 (1), pp. e2100137. Date of Electronic Publication: 2021 Oct 08.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Weinheim : Wiley-VCH Verlag, c2006-
MeSH Terms:: Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells*
Adipose Tissue ; Animals ; Injections, Intravenous ; Lung ; Mice ; Spheroids, Cellular
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Grant Information:: 2017 GSK Japan Research
Contributed Indexing:: Keywords: biodistribution; cell transplantation; mesenchymal stem cells; micromolding technique; multicellular spheroids
Entry Date(s):: Date Created: 20210928 Date Completed: 20220118 Latest Revision: 20220118
Update Code:: 20240105
DOI:: 10.1002/biot.202100137
PMID:: 34581003
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Background: Because of the excellent therapeutic potential, mesenchymal stem cells (MSCs) have been used as cell therapeutics for various diseases. However, the survival rate and duration of MSCs after transplantation are extremely low and short, respectively. To solve these problems, in this study, we prepared multicellular spheroids of MSCs and investigated their survival and function after intravenous injection in mice.
Methods and Results: The murine adipose-derived MSC line m17.ASC was cultured in agarose-based microwell plates to obtain size-controlled m17.ASC spheroids of an average diameter and cell number of approximately 170 μm and 1100 cells/spheroid, respectively. The intravenously injected m17.ASC spheroids mainly accumulated in the lung and showed a higher survival rate than suspended m17.ASC cells during the experimental period of 7 days. m17.ASC spheroids efficiently reduced the lipopolysaccharide-induced increase in plasma concentrations of interleukin-6 and tumor necrosis factor-α.
Conclusions: These results indicate that spheroid formation improved the pulmonary delivery and survival of MSCs, as well as their therapeutic potential against inflammatory pulmonary diseases.
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