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Tytuł:
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Detection of Major Human Herpesviruses in Iranian Patients with Suspected Encephalitis Using Multiplex Polymerase Chain Reaction.
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Autorzy:
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Golrokh Mofrad M; Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran.
Samie S; Pars Advanced and Minimally Invasive Medical Manners Research Center, Pars Hospital, Iran University of Medical Science, Tehran, Iran.
Lak R; Pars Advanced and Minimally Invasive Medical Manners Research Center, Pars Hospital, Iran University of Medical Science, Tehran, Iran.
Faghihloo E; Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
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Źródło:
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European neurology [Eur Neurol] 2022; Vol. 85 (2), pp. 142-147. Date of Electronic Publication: 2021 Sep 29.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: Basel, New York, Karger.
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MeSH Terms:
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Encephalitis*
Epstein-Barr Virus Infections*
Herpesviridae Infections*/diagnosis
DNA, Viral/analysis ; Herpesvirus 2, Human/genetics ; Herpesvirus 3, Human/genetics ; Herpesvirus 4, Human/genetics ; Humans ; Iran/epidemiology ; Multiplex Polymerase Chain Reaction
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Contributed Indexing:
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Keywords: Cytomegalovirus; Encephalitis; Epstein-Barr virus; Herpes simplex virus; Varicella zoster virus
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Substance Nomenclature:
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0 (DNA, Viral)
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Entry Date(s):
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Date Created: 20210929 Date Completed: 20220405 Latest Revision: 20220610
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Update Code:
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20240105
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DOI:
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10.1159/000519127
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PMID:
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34587615
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Background: The Herpesviridae family plays a significant etiological role in central nervous system viral infections during primary infection or reactivation from a latent form. Early detection is crucial because prescribing some antivirals can prevent severe side effects or life-threatening conditions.
Methods: In this study, 251 CSF specimens were collected from patients with clinical suspicion of viral encephalitis in Pars Hospital, Tehran, Iran. DNA was extracted, and a multiplex PCR was designed to investigate the presence of herpes simplex virus-1, herpes simplex virus-2, varicella zoster virus, Epstein-Barr virus, and cytomegalovirus.
Results: Overall, 59 cases of the 251 CSF samples were positive for multiplex PCR (23.5%). The most frequent positive findings were EBV and HSV, with a prevalence of 10.3% and 8.7% (5.5% HSV-1 and 3.1% HSV-2), respectively. Four co-infections were also seen in this study.
Conclusions: This multiplex PCR assay detects simultaneously different herpesviruses in CSF samples of patients with suspected encephalitis in 2 rounds of PCR amplification; therefore, it is a reliable and cost-saving diagnostic method for evaluating patients infected with herpesvirus with neurological disorders.
(© 2021 S. Karger AG, Basel.)
