Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Monitoring the Sialome on Human Immune Cells.

Tytuł:
Monitoring the Sialome on Human Immune Cells.
Autorzy:
O'Farrell LK; School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.
Fraser AD; Limerick General Hospitals, Limerick, Ireland.; School of Medicine, University of Limerick, Limerick, Ireland.
Davey GP; School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland. .
Źródło:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2370, pp. 323-329.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:
Leukocytes, Mononuclear*
N-Acetylneuraminic Acid*
Sialic Acids*/metabolism
Alkynes ; Click Chemistry ; Humans
References:
Boligan KF et al (2015) Cancer intelligence acquired (CIA): tumor glycosylation and sialylation codes dismantling antitumor defense. Cell Mol Life Sci 72(7):1231–1248. (PMID: 10.1007/s00018-014-1799-5)
Zhuo Y, Bellis SL (2011) Emerging role of alpha2,6-sialic acid as a negative regulator of galectin binding and function. J Biol Chem 286(8):5935–5941. (PMID: 10.1074/jbc.R110.191429)
Varki A, Angata T (2006) Siglecs—the major subfamily of I-type lectins. Glycobiology 16(1):1R–27R. (PMID: 10.1093/glycob/cwj008)
Xiao H et al (2016) Precision glycocalyx editing as a strategy for cancer immunotherapy. Proc Natl Acad Sci U S A 113(37):10304–10309. (PMID: 10.1073/pnas.1608069113)
Pfeifle R et al (2017) Regulation of autoantibody activity by the IL-23-TH17 axis determines the onset of autoimmune disease. Nat Immunol 18:104–113. (PMID: 10.1038/ni.3579)
Ohmi Y et al (2016) Sialylation converts arthritogenic IgG into inhibitors of collagen-induced arthritis. Nat Commun 7:11205. (PMID: 10.1038/ncomms11205)
Toscano MA et al (2007) Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death. Nat Immunol 8(8):825–834. (PMID: 10.1038/ni1482)
Clark MC, Baum LG (2012) T cells modulate glycans on CD43 and CD45 during development and activation, signal regulation, and survival. Ann N Y Acad Sci 1253:58–67. (PMID: 10.1111/j.1749-6632.2011.06304.x)
Speers AE, Cravatt BF (2004) Profiling enzyme activities in vivo using click chemistry methods. Chem Biol 11(4):535–546. (PMID: 10.1016/j.chembiol.2004.03.012)
Baskin JM et al (2007) Copper-free click chemistry for dynamic in vivo imaging. Proc Natl Acad Sci U S A 104(43):16793–16797. (PMID: 10.1073/pnas.0707090104)
Zaro BW, Bateman LA, Pratt MR (2011) Robust in-gel fluorescence detection of mucin-type O-linked glycosylation. Bioorg Med Chem Lett 21(17):5062–5066. (PMID: 10.1016/j.bmcl.2011.04.038)
Laughlin ST, Bertozzi CR (2007) Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Nat Protoc 2(11):2930–2944. (PMID: 10.1038/nprot.2007.422)
Saxon E et al (2002) Investigating cellular metabolism of synthetic azidosugars with the Staudinger ligation. J Am Chem Soc 124(50):14893–14902. (PMID: 10.1021/ja027748x)
Marshall A, Lichtman TJK, Seligsohn U, Kaushansky K, Prchal JT (2011) Williams hematology, 8th edn. McGraw-Hill, Pennsylvania.
Contributed Indexing:
Keywords: Click chemistry; Glycocalyx; Glycosylation; Immune cells; Inflammation; PBMCs; Sialome
Substance Nomenclature:
0 (Alkynes)
0 (Sialic Acids)
GZP2782OP0 (N-Acetylneuraminic Acid)
Entry Date(s):
Date Created: 20211006 Date Completed: 20211220 Latest Revision: 20211220
Update Code:
20240104
DOI:
10.1007/978-1-0716-1685-7_17
PMID:
34611878
Czasopismo naukowe
The sialome or display of sialic acids on the surface of human immune cells can vary according to immune response and activation state. Here, human peripheral blood mononuclear cells (PBMCs) were isolated and activated with anti-CD3 antibody and the cell surface sialome was quantified using a combination of click chemistry, confocal microscopy and flow cytometry techniques. Carbohydrate click chemistry was used to detect and measure the incorporation of an azido-m65odified sialic acid precursor molecule, N-acetylmannosamine (ManNaz) sugar into the PBMC surface sialome. Incorporation of sialic acid into the PBMC glycocalyx was visualized using copper-catalyzed click conjugation of Alexa 488 alkyne and confocal microscopy and further quantified using flow cytometry. The use of these methods indicate that regulating the sialome content on the surface of activated immune cells may be monitored during immunomodulatory responses and anti-inflammatory therapies.
(© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies