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Tytuł pozycji:

Three-dimensional electron paramagnetic resonance imaging of mice using ascorbic acid sensitive nitroxide imaging probes.

Tytuł:
Three-dimensional electron paramagnetic resonance imaging of mice using ascorbic acid sensitive nitroxide imaging probes.
Autorzy:
Sato-Akaba H; Department of Systems Innovation, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan.
Emoto MC; Department of Clinical Laboratory Science, School of Medical Technology, Health Sciences University of Hokkaido, Sapporo, Hokkaido, Japan.
Yamada KI; Faculty of Pharmaceutical Sciences, Physical Chemistry for Life Science Laboratory, Kyushu University, Fukuoka, Japan.
Koshino H; School of Dentistry, Health Sciences University of Hokkaido, Ishikari, Hokkaido, Japan.
Fujii HG; Advanced Research Promotion Center, Health Sciences University of Hokkaido, Ishikari, Hokkaido, Japan.
Źródło:
Free radical research [Free Radic Res] 2021 Oct; Vol. 55 (9-10), pp. 950-957. Date of Electronic Publication: 2021 Oct 25.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: London : Informa Healthcare
Original Publication: Yverdon, Switzerland : New York, NY : Harwood Academic ; distributed by STBS Ltd., c1994-
MeSH Terms:
Ascorbic Acid*
Nitrogen Oxides*
Animals ; Brain/diagnostic imaging ; Cyclic N-Oxides ; Electron Spin Resonance Spectroscopy/methods ; Mice ; Oxidation-Reduction
Contributed Indexing:
Keywords: EPR; Redox status; blood brain barrier; imaging; nitroxide; reactive oxygen species (ROS)
Substance Nomenclature:
0 (Cyclic N-Oxides)
0 (Nitrogen Oxides)
GFQ4MMS07W (nitroxyl)
PQ6CK8PD0R (Ascorbic Acid)
Entry Date(s):
Date Created: 20211011 Date Completed: 20220221 Latest Revision: 20220221
Update Code:
20240104
DOI:
10.1080/10715762.2021.1991918
PMID:
34632934
Czasopismo naukowe
Nitroxide compounds have been used as redox-sensitive imaging probes by electron paramagnetic resonance (EPR) for assessing oxidative stress in vivo . Fast redox reactions of nitroxide radicals are favorable for assessment of higher redox sensitivity; however, a variety of nitroxides have not been trialed for use as imaging probes due to their very rapid in vivo reduction, which cannot be captured at the slow operation speed of existing EPR imagers. To overcome this limitation, we improved our EPR system to provide a stable and highly sensitive imaging operation. We challenged the improved EPR imager to perform three-dimensional (3D) EPR imaging of mouse brain using two useful nitroxide imaging probes, 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol) and 2,6-dispiro-4',4"-dipyrane-piperidine-4-one-N-oxyl (DiPy). The second-order rate constant of DiPy with ascorbic acid is 10 times larger than that of Tempol. The improved EPR imager obtained clear 3D EPR images of mouse brain and demonstrated that Tempol could exist with an unpaired electron. The imager also successfully obtained 3D EPR images of mouse head after administration of DiPy. As 126 projections can be acquired in a period of 6 s, 3D EPR imaging can visualize the sequential process of DiPy entering the brain, being distributed within the brain, and being reduced within the brain. These improvements to the EPR imager will enable useful nitroxide imaging probes that were previously unsuitable as imaging probes due to their rapid reduction to be considered for use for sensitive redox assessment in an in vivo system.
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