Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2.

Tytuł:: Transcriptomics of chicken cecal tonsils and intestine after infection with low pathogenic avian influenza virus H9N2.
Autorzy:: Alqazlan N; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Emam M; Department of Human Genetics, McGill University, Montreal, QC, H3A 0E7, Canada.
Nagy É; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Bridle B; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Sargolzaei M; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.; Select Sires, Inc., Plain City, OH, 43064, USA.
Sharif S; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada. .
Źródło:: Scientific reports [Sci Rep] 2021 Oct 14; Vol. 11 (1), pp. 20462. Date of Electronic Publication: 2021 Oct 14.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Cecum/*immunology
Ileum/*immunology
Influenza A Virus, H9N2 Subtype/*immunology
Influenza in Birds/*immunology
Animals ; Cecum/metabolism ; Chickens ; Female ; Gene Expression Profiling ; Heat-Shock Proteins/genetics ; Heat-Shock Proteins/metabolism ; Ileum/metabolism ; Immunity, Innate ; Influenza in Birds/genetics ; Influenza in Birds/metabolism ; Interferons/genetics ; Interferons/metabolism ; RNA, Messenger
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Substance Nomenclature:: 0 (Heat-Shock Proteins)
0 (RNA, Messenger)
9008-11-1 (Interferons)
Entry Date(s):: Date Created: 20211015 Date Completed: 20220127 Latest Revision: 20230207
Update Code:: 20240104
PubMed Central ID:: PMC8517014
DOI:: 10.1038/s41598-021-99182-3
PMID:: 34650121
: Czasopismo naukowe

Pełny tekst

Influenza viruses cause severe respiratory infections in humans and birds, triggering global health concerns and economic burden. Influenza infection is a dynamic process involving complex biological host responses. The objective of this study was to illustrate global biological processes in ileum and cecal tonsils at early time points after chickens were infected with low pathogenic avian influenza virus (LPAIV) H9N2 through transcriptome analysis. Total RNA isolated from ileum and cecal tonsils of non-infected and infected layers at 12-, 24- and 72-h post-infection (hpi) was used for mRNA sequencing analyses to characterize differentially expressed genes and overrepresented pathways. Statistical analysis highlighted transcriptomic signatures significantly occurring 24 and 72 hpi, but not earlier at 12 hpi. Interferon (IFN)-inducible and IFN-stimulated gene (ISG) expression was increased, followed by continued expression of various heat-shock proteins (HSP), including HSP60, HSP70, HSP90 and HSP110. Some upregulated genes involved in innate antiviral responses included DDX60, MX1, RSAD2 and CMPK2. The ISG15 antiviral mechanism pathway was highly enriched in ileum and cecal tonsils at 24 hpi. Overall, most affected pathways were related to interferon production and the heat-shock response. Research on these candidate genes and pathways is warranted to decipher underlying mechanisms of immunity against LPAIV in chickens.
(© 2021. The Author(s).)

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