Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

High viral load positively correlates with thrombocytopenia and elevated haematocrit in dengue infected paediatric patients.

Tytuł:
High viral load positively correlates with thrombocytopenia and elevated haematocrit in dengue infected paediatric patients.
Autorzy:
Pathak B; Department of Molecular Medicine, School of Interdisciplinary Sciences & Technology, Jamia Hamdard, New Delhi 110062, India.
Chakravarty A; Department of Paediatrics, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, New Delhi, India.
Krishnan A; Department of Molecular Medicine, School of Interdisciplinary Sciences & Technology, Jamia Hamdard, New Delhi 110062, India. Electronic address: .
Źródło:
Journal of infection and public health [J Infect Public Health] 2021 Nov; Vol. 14 (11), pp. 1701-1707. Date of Electronic Publication: 2021 Oct 07.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Oxford : Elsevier, 2008-
MeSH Terms:
Dengue*/epidemiology
Dengue Virus*
Thrombocytopenia*/epidemiology
Antibodies, Viral ; Child ; Enzyme-Linked Immunosorbent Assay ; Hematocrit ; Humans ; Immunoglobulin M ; Viral Load
Contributed Indexing:
Keywords: Biomarker; Dengue; Haematocrit; NS1; Platelets; Viral load
Substance Nomenclature:
0 (Antibodies, Viral)
0 (Immunoglobulin M)
Entry Date(s):
Date Created: 20211016 Date Completed: 20211110 Latest Revision: 20211110
Update Code:
20240104
DOI:
10.1016/j.jiph.2021.10.002
PMID:
34655984
Czasopismo naukowe
Background: Dengue fever is one of the major viral diseases worldwide transmitted by mosquitoes. Depending on the severity of disease it can range from mild fever to severe fatal cases. Rapid decline of platelet levels is one of indicators of clinical worsening. The role of viral factors in dengue pathogenesis and correlation with clinical and laboratory parameters remain unclear.
Methods: Between September 2017 to December 2018, 102 dengue confirmed paediatric cases were analysed for various viral and host parameters. Based on symptoms, they were classified into dengue without warning signs (DOS), dengue with warning signs (DWS) and severe dengue (SD) as per 2009 WHO classification. Quantitative analysis of NS1, IgM and IgG in were done by ELISA. IgM/IgG ratio revealed primary or secondary dengue infection. Serotyping of virus in serum was done by nested multiplex RT-PCR. Viral load (VL) was determined by quantitative real time polymerase chain reaction. Association between VL and NS1 in patient sera with clinical and laboratory parameters was statistically analysed.
Results: It was found that disease severity (as per 2009 WHO classification) significantly associated with secondary dengue infection. DENV3 was found to be the only serotype detected. The present study reports neither NS1 nor VL significantly associated with disease severity or type of infection (primary or secondary). However, VL positively correlated with haematocrit (p < 0.05). Viral load above 10 6 copies/mL was found in 61% of patients. Further, high viral load (>10 6 copies/mL) negatively correlated with platelet levels (p < 0.05).
Conclusion: Thus, viral load could be an important predictive parameter in dengue related severe symptoms like thrombocytopenia and elevated hematocrit when it goes above a certain threshold (>10 6 copies/ mL).
(Copyright © 2021. Published by Elsevier Ltd.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies