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Tytuł:
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Lumpy skin disease outbreaks in Egypt during 2017-2018 among sheeppox vaccinated cattle: Epidemiological, pathological, and molecular findings.
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Autorzy:
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Rouby SR; Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, Egypt.
Safwat NM; Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, Egypt.
Hussein KH; Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, Egypt.
Abdel-Ra'ouf AM; Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
Madkour BS; Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
Abdel-Moneim AS; Microbiology Department, College of Medicine, Taif University, Al-Taif, Saudi Arabia.
Hosein HI; Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, Egypt.
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Źródło:
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PloS one [PLoS One] 2021 Oct 20; Vol. 16 (10), pp. e0258755. Date of Electronic Publication: 2021 Oct 20 (Print Publication: 2021).
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: San Francisco, CA : Public Library of Science
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MeSH Terms:
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Disease Outbreaks/*veterinary
Lumpy Skin Disease/*epidemiology
Lumpy skin disease virus/*classification
Vaccination/*veterinary
Viral Proteins/*genetics
Animals ; Cattle ; Egypt/epidemiology ; Evolution, Molecular ; Female ; Lumpy Skin Disease/mortality ; Lumpy Skin Disease/virology ; Lumpy skin disease virus/genetics ; Lumpy skin disease virus/isolation & purification ; Male ; Mortality ; Phylogeny ; Sequence Analysis, DNA
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References:
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Substance Nomenclature:
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0 (Viral Proteins)
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Entry Date(s):
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Date Created: 20211020 Date Completed: 20211125 Latest Revision: 20211125
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Update Code:
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20240105
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PubMed Central ID:
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PMC8528327
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DOI:
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10.1371/journal.pone.0258755
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PMID:
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34669748
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The General Organization of the Veterinary Services in Egypt has adopted a sheeppox vaccination policy to control lumpy skin disease (LSD) in cattle. Over the course of the last two years, recurrent outbreaks were reported, with animals showing severe clinical signs and consequentially higher fatalities than that of cases reported in previous LSD outbreaks. A total of 1050 cattle showing typical clinical signs suggestive of LSD were clinically and pathologically investigated during 2017-2018. Skin nodules were collected and lumpy skin disease virus (LSDV) was screened in collected skin samples using PCR for the RPO-30 gene. Furthermore, the entire P32 protein coding gene was sequenced. Histopathology and immunohistochemistry of the skin nodules were also conducted. The obtained results showed an overall mortality rate of 6.86%. LSDV was confirmed in all the examined nodules as evidenced by immunohistochemistry and positive PCR amplification of the RPO30 gene. Sequencing analysis of the P32 gene revealed a highly conserved nature and genetic stability of the LSDV. The results of the present study show that the current vaccination protocol was not effective for a multitude of reasons. These results also serve as evidence for a strong recommendation of an amendment of homologous vaccine use aside from a complete coverage of cattle populations in order to reduce the incidence of LSD among cattle population in Egypt.
Competing Interests: The authors have declared that no competing interests exist.