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Tytuł:
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UPLC-MS/MS analysis and biological activity of the potato cyst nematode hatching stimulant, solanoeclepin A, in the root exudate of Solanum spp.
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Autorzy:
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Guerrieri A; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Floková K; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Vlaar LE; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Schilder ML; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Kramer G; Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Chojnacka A; Biomolecular System Analytics, Van't Hoff Institute for Molecular Sciences (HIMS), University of Amsterdam, Amsterdam, The Netherlands.
van Dijk YR; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands.
Bouwmeester HJ; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands. .
Dong L; Plant Hormone Biology Group, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, The Netherlands. .
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Źródło:
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Planta [Planta] 2021 Nov 02; Vol. 254 (6), pp. 112. Date of Electronic Publication: 2021 Nov 02.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Berlin, New York, Springer-Verlag [etc.]
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MeSH Terms:
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Solanum tuberosum*/chemistry
Tylenchoidea*/pathogenicity
Bridged-Ring Compounds/*chemistry
Hexanes/*chemistry
Plant Diseases/*parasitology
Animals ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Exudates and Transudates ; Plant Breeding ; Plant Roots/chemistry ; Tandem Mass Spectrometry
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References:
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Phytochemistry. 2014 Sep;105:147-57. (PMID: 24947339)
Biochem J. 1949;45(5):524-8. (PMID: 15396617)
Front Plant Sci. 2020 Jun 10;11:649. (PMID: 32587595)
Biochem J. 1949;45(5):513-9, 2 pl. (PMID: 15396615)
Plant Methods. 2020 Sep 17;16:125. (PMID: 32963580)
Phytochemistry. 2003 Mar;62(6):959-69. (PMID: 12590123)
Talanta. 2013 Aug 15;112:85-94. (PMID: 23708542)
Plant Biotechnol (Tokyo). 2020 Sep 25;37(3):319-325. (PMID: 33088195)
J Agric Food Chem. 2008 Aug 27;56(16):6949-58. (PMID: 18616273)
Nat Chem. 2011 Jun;3(6):484-8. (PMID: 21602865)
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Biochem J. 1949;45(5):520-4. (PMID: 15396616)
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Grant Information:
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670211 International ERC_ European Research Council; 793795 h2020 marie skłodowska-curie actions; 16873 dutch research council nwo-ttw
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Contributed Indexing:
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Keywords: Analytical chemistry; Eclepins; Hatching assay; Solid phase extraction (SPE); Tomato root exudates
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Substance Nomenclature:
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0 (Bridged-Ring Compounds)
0 (Hexanes)
0 (solanoeclepin A)
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Entry Date(s):
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Date Created: 20211102 Date Completed: 20211104 Latest Revision: 20220531
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Update Code:
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20240105
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PubMed Central ID:
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PMC8563560
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DOI:
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10.1007/s00425-021-03766-2
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PMID:
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34727239
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Main Conclusion: Solanoeclepin A is a hatching stimulant for potato cyst nematode in very low (pM) concentrations. We report a highly sensitive method for the analysis of SolA in plant root exudates using UHPLC-MS/MS and show that there is considerable natural variation in SolA production in Solanum spp. corresponding with their hatching inducing activity. Potato cyst nematode (PCN) is a plant root sedentary endoparasite, specialized in the infection of solanaceous species such as potato (Solanum tuberosum) and tomato (Solanum lycopersicum). Earlier reports (Mulder et al. in Hatching agent for the potato cyst nematode, Patent application No. PCT/NL92/00126, 1996; Schenk et al. in Croat Chem Acta 72:593-606, 1999) showed that solanoeclepin A (SolA), a triterpenoid metabolite that was isolated from the root exudate of potato, induces the hatching of PCN. Its low concentration in potato root exudate has hindered progress in fully understanding its hatching inducing activity and exploitation in the control of PCN. To further investigate the role of SolA in hatching of PCN, the establishment of a highly sensitive analytical method is a prerequisite. Here we present the efficient single-step extraction and UHPLC-MS/MS based analysis for rapid determination of SolA in sub-nanomolar concentrations in tomato root exudate. This method was used to analyze SolA production in different tomato cultivars and related solanaceous species, including the trap crop Solanum sisymbriifolium. Hatching assays with PCN, Globodera pallida, with root exudates of tomato genotypes revealed a significant positive correlation between SolA concentration and hatching activity. Our results demonstrate that there is natural variation in SolA production within solanaceous species and that this has an effect on PCN hatching. The analytical method we have developed can potentially be used to support breeding for crop genotypes that induce less hatching and may therefore display reduced infection by PCN.
(© 2021. The Author(s).)