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Tytuł pozycji:

A highly selective ATP-responsive biomimetic nanochannel based on smart copolymer.

Tytuł:
A highly selective ATP-responsive biomimetic nanochannel based on smart copolymer.
Autorzy:
Liu Q; School of Chemistry and Molecular Engineering, Engineering Research Center of Nanophotonics and Advanced Instrument, Ministry of Education, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People's Republic of China.
Ding S; School of Chemistry and Molecular Engineering, Engineering Research Center of Nanophotonics and Advanced Instrument, Ministry of Education, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People's Republic of China; School of Pharmacy, Nantong University, 19 Qixiu Road, Nantong, 226001, People's Republic of China.
Gao R; Department of Chemistry, University of Utah, Salt Lake City, UT, 84112, USA.
Shi G; School of Chemistry and Molecular Engineering, Engineering Research Center of Nanophotonics and Advanced Instrument, Ministry of Education, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People's Republic of China. Electronic address: .
Zhu A; School of Chemistry and Molecular Engineering, Engineering Research Center of Nanophotonics and Advanced Instrument, Ministry of Education, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People's Republic of China. Electronic address: .
Źródło:
Analytica chimica acta [Anal Chim Acta] 2021 Dec 15; Vol. 1188, pp. 339167. Date of Electronic Publication: 2021 Oct 12.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Amsterdam : Elsevier
Original Publication: Amsterdam.
MeSH Terms:
Adenosine Triphosphate*
Biomimetics*
Binding Sites ; Nucleotides ; Sulfonylurea Receptors
Contributed Indexing:
Keywords: ATP-sensitive potassium channel; Nanochannels; Smart copolymer
Substance Nomenclature:
0 (Nucleotides)
0 (Sulfonylurea Receptors)
8L70Q75FXE (Adenosine Triphosphate)
Entry Date(s):
Date Created: 20211119 Date Completed: 20211122 Latest Revision: 20211122
Update Code:
20240105
DOI:
10.1016/j.aca.2021.339167
PMID:
34794583
Czasopismo naukowe
ATP-sensitive potassium (K ATP ) channels couple intracellular metabolism to the electrical activity by regulating K + flux across the plasma membrane, thus playing an important role in both normal and pathophysiology. To understand the mechanism of ATP regulating biological ion channels, developing an ATP-responsive artificial nanochannel is an appealing but challenging topic because K ATP channel is a heteromultimer of two subunits (potassium channel subunit (Kir6.x) and sulfonylurea receptor (SUR)) and exhibit dynamic functions with adjustability and reversibility. Inspired by the structure of K ATP channels, we designed a smart copolymer modified nanochannel that may address the challenge. In the tricomponent poly(N-isopropylacrylamide) (PNIPAAm, PNI)-based copolymer system, phenylthiourea was used to bind the phosphate units of nucleotides and phenylboronic acid was introduced to combine the pentose ring of the nucleoside unit. Besides, a -COOH group with electron-withdrawing property was added into the phenylthiourea units, which may promote the hydrogen-bond-donating ability of thiourea. Specially, the smart copolymer not only provided static binding sites for recognition but also translated the recognition of ATP into their dynamic conformational transitions by changing the hydrogen-bonding environments surrounding PNIPAAm chains, thus achieving the gating function of nanochannel, which resembled the integration and coordination of Kir6.x and SUR units in active K ATP . The ATP-regulated ion channel exhibited excellent stability and reversibility. This study is the first example showing how to learn from nature to assemble the ATP-responsive artificial nanochannel and demonstrate the possible mechanism of ATP gating.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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