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Tytuł:
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Influence of conceptus presence and preovulatory estradiol exposure on uterine gene transcripts and proteins around maternal recognition of pregnancy in beef cattle.
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Autorzy:
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Northrop-Albrecht EJ; Department of Animal Science, South Dakota State University, Brookings, SD, USA.
Rich JJJ; Department of Animal Science, South Dakota State University, Brookings, SD, USA.
Cushman RA; USDA, Agricultural Research Service, Roman L. Hruska US Meat Animal Research Center, Clay Center, NE, USA.
Yao R; Department of Mathematics and Statistics, South Dakota State University, Brookings, SD, USA.
Ge X; Department of Mathematics and Statistics, South Dakota State University, Brookings, SD, USA.
Perry GA; Department of Animal Science, South Dakota State University, Brookings, SD, USA. Electronic address: .
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Źródło:
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Molecular and cellular endocrinology [Mol Cell Endocrinol] 2022 Jan 15; Vol. 540, pp. 111508. Date of Electronic Publication: 2021 Nov 18.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Limerick : North Holland Publishing
Original Publication: Amsterdam, North-Holland.
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MeSH Terms:
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Cattle*/genetics
Cattle*/physiology
Pregnancy, Animal*/genetics
Pregnancy, Animal*/psychology
Embryo, Mammalian/*physiology
Perception/*physiology
Uterus/*metabolism
Animals ; Embryo, Mammalian/diagnostic imaging ; Embryonic Development/physiology ; Endometrium/metabolism ; Estradiol/pharmacology ; Female ; Follicular Phase/drug effects ; Follicular Phase/physiology ; Gene Expression Regulation ; Pregnancy ; Pregnancy Proteins/genetics ; Pregnancy Proteins/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Ultrasonography, Prenatal/veterinary ; Uterus/diagnostic imaging
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Contributed Indexing:
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Keywords: Conceptus; Maternal recognition of pregnancy; Proteomics; Transcriptomics; Uterus
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Substance Nomenclature:
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0 (Pregnancy Proteins)
0 (RNA, Messenger)
4TI98Z838E (Estradiol)
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Entry Date(s):
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Date Created: 20211120 Date Completed: 20220328 Latest Revision: 20220328
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Update Code:
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20240105
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DOI:
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10.1016/j.mce.2021.111508
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PMID:
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34800604
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The uterine environment must provide sufficient endocrine conditions and nutrients for pregnancy maintenance and conceptus survival. The objective of this study was to determine the effects of preovulatory estradiol and conceptus presence on uterine transcripts and uterine luminal fluid (ULF) proteins. Beef cows/heifers were synchronized and artificially inseminated (d 0). Uteri were flushed (d 16); conceptuses and endometrial biopsies were collected. Total cellular RNA was extracted from endometrium for RNA sequencing and RT-PCR validation. There were two independent ULF pools made for each of the following groups: highE2/conceptus, highE2/noconceptus, lowE2/conceptus, and lowE2/noconceptus that were analyzed using the 2D LC-MS/MS based iTRAQ method. There were 64 differentially expressed genes (DEGs) and 77 differentially expressed proteins (DEPs) in common among the highE2/conceptus vs highE2/noconceptus and lowE2/conceptus vs lowE2/noconceptus groups. In summary, the interaction between preovulatory estradiol and the conceptus induces the expression of genes, proteins, and pathways necessary for pregnancy.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
