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Tytuł:
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Efficacy of Cyanotis tuberosa (Roxb.) Schult. &Schult. f. root tubers' active fraction as anti-diabetic, antihyperlipidemic and antioxidant in Streptozotocin-induced diabetic rats.
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Autorzy:
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Vedasree N; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Peddanna K; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Rajasekhar A; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
ParthaSarathi C; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Munirajeswari P; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Sireesha Y; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Chippada AR; Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India. Electronic address: .
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Źródło:
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Journal of ethnopharmacology [J Ethnopharmacol] 2022 Mar 01; Vol. 285, pp. 114856. Date of Electronic Publication: 2021 Nov 20.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
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MeSH Terms:
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Antioxidants/*pharmacology
Commelinaceae/*chemistry
Hypoglycemic Agents/*therapeutic use
Hypolipidemic Agents/*therapeutic use
Plant Extracts/*therapeutic use
Plant Roots/*chemistry
Animals ; Antioxidants/chemistry ; Biomarkers/blood ; Blood Glucose/drug effects ; Diabetes Mellitus, Experimental ; Glucose Tolerance Test ; Hypoglycemic Agents/administration & dosage ; Hypolipidemic Agents/chemistry ; Kidney/drug effects ; Kidney/metabolism ; Lipid Metabolism/drug effects ; Liver/drug effects ; Liver/metabolism ; Male ; Phytotherapy ; Plant Extracts/chemistry ; Rats ; Rats, Wistar ; Toxicity Tests
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Contributed Indexing:
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Keywords: Antihyperglycemic activity; Antioxidant status; Cyanotis tuberosa; Diabetes mellitus; Streptozotocin
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Substance Nomenclature:
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0 (Antioxidants)
0 (Biomarkers)
0 (Blood Glucose)
0 (Hypoglycemic Agents)
0 (Hypolipidemic Agents)
0 (Plant Extracts)
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Entry Date(s):
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Date Created: 20211122 Date Completed: 20220217 Latest Revision: 20220217
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Update Code:
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20240105
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DOI:
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10.1016/j.jep.2021.114856
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PMID:
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34808300
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Ethnopharmacological Relevance: Cyanotis tuberosa (Roxb.) Schult. &Schult.f. is traditionally used as ethnomedicine for curing several ailments like diabetes, liver problems, ulcers, etc. OBJECTIVE: The present study was designed to evaluate the anti-diabetic potential of Cyanotis tuberosa root tubers (CTRT)in Streptozotocin (STZ) induced diabetic rats.
Materials and Methods: Anti-hyperglycemic activity of hexane extract of CTRT was investigated in diabetic rats. Silica gel chromatography was used to fractionate the hexane extract and the fraction's antihyperglycemic activity was checked in diabetic rats. Effects of long-term (30 days) treatment with an active fraction (CTAF) were evaluated in diabetic rats for 30 days by measurement of body weights, glycemic control, insulin levels, HbA1c, and serum and tissue lipid profiles. Lipid peroxide levels and antioxidant status were measured in the liver and kidney. Hepatic and Renal functional markers were also measured. Phytochemical characterization of CTAF was carried out by LC-ESI-MS/MS analysis.
Results: Hexane extract of CTRT at a dose of 750 mg/kg b.w produced significant antihyperglycemic activity in diabetic rats whereas CTAF has produced maximum antihyperglycemic activity at the dose of 75 mg/kg b.w. Following long-term treatment with CTAF in diabetic rats, significant improvement in glycemic control, (HbA1c) along with decreased insulin resistance (HOMA-IR), increase in body weights, and plasma insulin were observed. Also, CTAF ameliorated the serum and tissue lipid profiles. In addition, CTAF suppressed lipid peroxidation and restored the activities of antioxidant enzymes in the liver and kidney to normal levels. Further, CTAF reversed the liver and kidney functional markers to normalcy. LC-ESI-MS/MS analysis revealed the presence of 7 different phytoconstituents.
Conclusion: This study confirmed that CTAF exerts antidiabetic effects in diabetic rats by improving insulin secretion, glycemic control, and restoring functional activities of the liver and kidney. Our results suggest that root tubers of Cyanotis tuberosa can be used as a complementary or alternative agent for the treatment of diabetes mellitus.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
