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Tytuł:
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Screening of bioactive ingredients of Tsantan Sumtang in ameliorating H9c2 cells injury.
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Autorzy:
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Zhou Y; College of Eco-environmental Engineering, Qinghai University, Xining, 810016, PR China. Electronic address: .
Li Z; Research Center for High Altitude Medicine, Qinghai University, Xining, 810016, PR China.
Zhang D; College of Eco-environmental Engineering, Qinghai University, Xining, 810016, PR China.
Zhang B; College of Eco-environmental Engineering, Qinghai University, Xining, 810016, PR China.
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Źródło:
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Journal of ethnopharmacology [J Ethnopharmacol] 2022 Mar 01; Vol. 285, pp. 114854. Date of Electronic Publication: 2021 Nov 19.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
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MeSH Terms:
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Medicine, Tibetan Traditional*
Myoblasts/*drug effects
Myocardial Ischemia/*drug therapy
Polyphenols/*pharmacology
Animals ; Antibiotics, Antineoplastic/toxicity ; Apigenin/administration & dosage ; Apigenin/chemistry ; Apigenin/pharmacology ; Cell Line ; Dose-Response Relationship, Drug ; Doxorubicin/toxicity ; Eugenol/administration & dosage ; Eugenol/analogs & derivatives ; Eugenol/chemistry ; Eugenol/pharmacology ; Hydrogen Peroxide/toxicity ; Myoblasts/physiology ; Phytotherapy ; Polyphenols/blood ; Polyphenols/chemistry ; Polyphenols/pharmacokinetics ; Quercetin/administration & dosage ; Quercetin/analogs & derivatives ; Quercetin/chemistry ; Quercetin/pharmacology ; Rats ; Rats, Sprague-Dawley
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Contributed Indexing:
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Keywords: Bioactive ingredients; H9c2 cells; Polyphenols; Serum pharmacochemistry; Tsantan sumtang
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Substance Nomenclature:
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0 (Antibiotics, Antineoplastic)
0 (Polyphenols)
2Y8906LC5P (quercitrin)
3T8H1794QW (Eugenol)
5M0MWY797U (isoeugenol)
7V515PI7F6 (Apigenin)
80168379AG (Doxorubicin)
9IKM0I5T1E (Quercetin)
BBX060AN9V (Hydrogen Peroxide)
KTQ9R9MS0Q (isovitexin)
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Entry Date(s):
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Date Created: 20211122 Date Completed: 20220217 Latest Revision: 20220217
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Update Code:
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20240105
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DOI:
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10.1016/j.jep.2021.114854
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PMID:
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34808301
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Ethnopharmacological Relevance: Tsantan Sumtang (TS), a traditional Tibetan medicine, has been used in the clinic for the treatment of myocardial ischemia (MI) for ages, however, the bioactive ingredients that are responsible for improving MI remain unknown.
Aim of the Study: This study investigated the chemical components of TS and their medicinal efficacies at cell levels, in order to expound the bioactive ingredients in TS.
Materials and Methods: First, a response-surface methodology was employed to determine the optimum ethanol reflux extraction process of polyphenols in TS (PTS) due to their close correlation with MI improvement. Second, a serum pharmacochemistry technique was used to analyze the compounds of PTS absorbed into the blood of rats. Third, hypoxia-, H 2 O 2 -, and adriamycin (ADM)-induced H9c2 cell injury models were used to investigate the cardioprotective effects of these compounds in vitro. Fourth, protective effects of isovitexin, quercitrin, and isoeugenol on mitochondrial function were further tested.
Results: The optimum extraction conditions for obtaining PTS were an ethanol concentration of 78.22%, an extraction time of 67.4 min, and a material-liquid ratio of 1:72.60 mL/g. Serum pharmacochemistry analysis detected 21 compounds, of which 11 compounds were always present in the blood within 5 h. Cytotoxicity and the protective effect of 11 compounds in hypoxia-, H 2 O 2 -, and ADM-induced H9c2 cell injury models shown that isovitexin, quercitrin, and isoeugenol had almost no cytotoxicity, and they could elevate the survival rate in injured H9c2 cells. Furthermore, isovitexin, quercitrin, and isoeugenol could decrease mitochondrial reactive oxygen species (ROS) releasion, inhibite mitochondrial permeability transition pore (mPTP) opening, ameliorate the change of mitochondrial membrane potential (MMP) to exert mitochondrial protection effect.
Conclusion: Isovitexin, quercitrin, and isoeugenol exhibited cardioprotective effect at cell levles, these three compounds might be the bioactive ingredients in TS. These findings elucidate the pharmacodynamic substances and mechanisms of TS, guiding its clinical use.
(Copyright © 2021. Published by Elsevier B.V.)
