Circular RNA circ&#95;0023404 serves as a miR-636 sponge to promote malignant behaviors in cervical cancer cells through upregulation of CYP2S1. - OpacWWW

Szczegóły
Abstrakt

Tytuł:: Circular RNA circ_0023404 serves as a miR-636 sponge to promote malignant behaviors in cervical cancer cells through upregulation of CYP2S1.
Autorzy:: Guo WW; Department of Gynecology and Obstetrics, Dalian Municipal Women and Children's Medical Center (Group), Dalian, China.
Feng MM; Department of Pathology, Dalian Liaoyu Hospital, Dalian, China.
Li SF; Department of Gynecology and Obstetrics, Dalian hospital of Shengjing Hospital Affiliated to China Medical University, Dalian, China.
Wei LH; Department of Gynecology and Obstetrics, Siping Maternal and Child Health Care Hospital, Siping, China.
Źródło:: The Kaohsiung journal of medical sciences [Kaohsiung J Med Sci] 2022 Mar; Vol. 38 (3), pp. 218-229. Date of Electronic Publication: 2021 Nov 26.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2019- : [Kaohsiung City, Taiwan] : Department of Public Health, Kaohsiung Medical University
Original Publication: [Kaohsiung City, Taiwan, Republic of China : Kaohsiung Medical College, 1996-
MeSH Terms:: Gene Expression Regulation, Neoplastic*
Up-Regulation*
Cytochrome P-450 Enzyme System/*genetics
MicroRNAs/*genetics
RNA, Circular/*genetics
Uterine Cervical Neoplasms/*genetics
Female ; Humans
References:: Siegel RL, Miller KD, Jemal A. Cancer statistics, 2020. CA Cancer J Clin. 2020;70(1):7-30.
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394-424.
Walker TY, Elam-Evans LD, Yankey D, Markowitz LE, Williams CL, Fredua B, et al. National, regional, state, and selected local area vaccination coverage among adolescents aged 13-17 years: United States, 2018. MMWR Morb Mortal Wkly Rep. 2019;68(33):718-23.
Tsikouras P, Zervoudis S, Manav B, Tomara E, Iatrakis G, Romanidis C, et al. Cervical cancer: screening, diagnosis and staging. J BUON. 2016;21(2):320-5.
ENCODE Project Consortium, Birney E, Stamatoyannopoulos JA, Dutta A, Guigó R, Gingeras TR, et al. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature. 2007;447(7146):799-816.
Hentze MW, Preiss T. Circular RNAs: splicing's enigma variations. EMBO J. 2013;32(7):923-5.
Zhang HD, Jiang LH, Sun DW, Hou JC, Ji ZL. CircRNA: a novel type of biomarker for cancer. Breast Cancer. 2018;25(1):1-7.
Kristensen LS, Hansen TB, Venø MT, Kjems J. Circular RNAs in cancer: opportunities and challenges in the field. Oncogene. 2018;37(5):555-65.
Lux S, Bullinger L. Circular RNAs in cancer. Adv Exp Med Biol. 2018;1087:215-30.
Qu S, Liu Z, Yang X, Zhou J, Yu H, Zhang R, et al. The emerging functions and roles of circular RNAs in cancer. Cancer Lett. 2018;414:301-9.
Tang Q, Chen Z, Zhao L, Xu H. Circular RNA hsa_circ_0000515 acts as a miR-326 sponge to promote cervical cancer progression through up-regulation of ELK1. Aging (Albany NY). 2019;11(22):9982-99.
Cai H, Zhang P, Xu M, Yan L, Liu N, Wu X. Circular RNA hsa_circ_0000263 participates in cervical cancer development by regulating target gene of miR-150-5p. J Cell Physiol. 2019;234(7):11391-400.
Liu C, Zhang Z, Qi D. Circular RNA hsa_circ_0023404 promotes proliferation, migration and invasion in non-small cell lung cancer by regulating miR-217/ZEB1 axis. Onco Targets Ther. 2019;12:6181-9.
Wang T, Hao Z, Liu C, Yuan L, Li L, Yin M, et al. LEF1 mediates osteoarthritis progression through circRNF121/miR-665/MYD88 axis via NF-кB signaling pathway. Cell Death Dis. 2020;11(7):598.
Panda AC. Circular RNAs act as miRNA sponges. Adv Exp Med Biol. 2018;1087:67-79.
Anastasiadou E, Jacob LS, Slack FJ. Non-coding RNA networks in cancer. Nat Rev Cancer. 2018;18(1):5-18.
Yi Y, Liu Y, Wu W, Wu K, Zhang W. Reconstruction and analysis of circRNA-miRNA-mRNA network in the pathology of cervical cancer. Oncol Rep. 2019;41(4):2209-25.
Song J, Shi W, Gao Z, Liu X, Wang W. Downregulation of circRNA_100876 inhibited progression of NSCLC in vitro via targeting miR-636. Technol Cancer Res Treat. 2020;19:1533033820951817.
Jang JY, Lee YS, Jeon YK, Lee K, Jang JJ, Kim CW. ANT2 suppression by shRNA restores miR-636 expression, thereby downregulating Ras and inhibiting tumorigenesis of hepatocellular carcinoma. Exp Mol Med. 2013;45(1):e3.
Hu QL, Xu ZP, Lan YF, Li B. miR-636 represses cell survival by targeting CDK6/Bcl-2 in cervical cancer. Kaohsiung J Med Sci. 2020;36(5):328-35.
Li Y, Su X, Feng C, Liu S, Guan H, Sun Y, et al. CYP2S1 is a synthetic lethal target in BRAFV600E-driven thyroid cancers. Signal Transduct Target Ther. 2020;5(1):191.
Cabrera-Cano A, Dávila-Borja VM, Juárez-Méndez S, Marcial-Quino J, Gómez-Manzo S, Castillo-Rodríguez RA. Hypoxia as a modulator of cytochromes P450: overexpression of the cytochromes CYP2S1 and CYP24A1 in human liver cancer cells in hypoxia. Cell Biochem Funct. 2021;39(4):478-87.
Sekar S, Geiger P, Cuyugan L, Boyle A, Serrano G, Beach TG, et al. Identification of circular RNAs using RNA sequencing. J Vis Exp. 2019;153:e59981.
Meng S, Zhou H, Feng Z, Xu Z, Tang Y, Li P, et al. CircRNA: functions and properties of a novel potential biomarker for cancer. Mol Cancer. 2017;16(1):94.
Arnaiz E, Sole C, Manterola L, Iparraguirre L, Otaegui D, Lawrie CH. CircRNAs and cancer: biomarkers and master regulators. Semin Cancer Biol. 2019;58:90-9.
Chaichian S, Shafabakhsh R, Mirhashemi SM, Moazzami B, Asemi Z. Circular RNAs: a novel biomarker for cervical cancer. J Cell Physiol. 2020;235(2):718-24.
Huang H, Chen YF, Du X, Zhang C. Identification and characterization of tumorigenic circular RNAs in cervical cancer. Cell Signal. 2020;73:109669.
Guo J, Chen M, Ai G, Mao W, Li H, Zhou J. Hsa_circ_0023404 enhances cervical cancer metastasis and chemoresistance through VEGFA and autophagy signaling by sponging miR-5047. Biomed Pharmacother. 2019;115:108957.
Thomas LF, Saetrom P. Circular RNAs are depleted of polymorphisms at microRNA binding sites. Bioinformatics. 2014;30(16):2243-6.
Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, et al. Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 2013;495(7441):333-8.
He Q, Huang L, Yan D, Bi J, Yang M, Huang J, et al. CircPTPRA acts as a tumor suppressor in bladder cancer by sponging miR-636 and upregulating KLF9. Aging (Albany NY). 2019;11(23):11314-28.
Zhang J, Zhao X, Zhang J, Zheng X, Li F. Circular RNA hsa_circ_0023404 exerts an oncogenic role in cervical cancer through regulating miR-136/TFCP2/YAP pathway. Biochem Biophys Res Commun. 2018;501(2):428-33.
Saarikoski ST, Rivera SP, Hankinson O, Husgafvel-Pursiainen K. CYP2S1: a short review. Toxicol Appl Pharmacol. 2005;207:62-9.
Szaefer H, Licznerska B, Cykowiak M, Baer-Dubowska W. Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes. Pharmacol Rep. 2019;71(6):1001-5.
Contributed Indexing:: Keywords: CYP2S1; cervical cancer; circ_0023404; miR-636
Substance Nomenclature:: 0 (MicroRNAs)
0 (RNA, Circular)
9035-51-2 (Cytochrome P-450 Enzyme System)
EC 1.14.- (CYP2S1 protein, human)
Entry Date(s):: Date Created: 20211126 Date Completed: 20220328 Latest Revision: 20220328
Update Code:: 20240104
DOI:: 10.1002/kjm2.12478
PMID:: 34825467
: Czasopismo naukowe

Full Text Finder

Cervical cancer is the most common malignant gynecological tumor. Circular RNA (circRNA) circ_0023404 is reported to be upregulated in cervical cancer cells. This aim is to explore the role and mechanism of circ_0023404 in cervical cancer. circ_0023404, microRNA-636 (miR-636), and cytochrome P450 2S1 (CYP2S1) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Cell proliferation, migration, invasion, and apoptosis were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, 5-ethynyl-2'-deoxyuridine (EDU) assay, colony formation assay, transwell assay, and cytometry assay. Protein levels of cyclin D1, matrix metallopeptidase 9 (MMP9), Bcl-2-associated X protein (Bax), and CYP2S1 were examined by western blot assay. The binding relationship between miR-636 and circ_0023404 or CYP2S1 was predicted by Circinteractome or targetscan, and then verified by a dual-luciferase reporter assay and RNA pull-down assay. circ_0023404 and CYP2S1 expression were increased, and miR-636 was decreased in cervical cancer tissues and cells. Moreover, circ_0023404 knockdown could repress proliferation, migration, invasion, and promote apoptosis of cervical cancer cells in vitro. Mechanically, circ_0023404 could regulate CYP2S1 expression by sponging miR-636. circ_0023404 silencing could attenuate the progression of cervical cancer cells partly by targeting the miR-636/CYP2S1 axis, hinting at a promising therapeutic target for cervical cancer.
(© 2021 The Authors. The Kaohsiung Journal of Medical Sciences published by John Wiley & Sons Australia on behalf of Kaohsiung Medical University.)

Informacja

Circular RNA circ_0023404 serves as a miR-636 sponge to promote malignant behaviors in cervical cancer cells through upregulation of CYP2S1.