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Tytuł:
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Porcine iPSC Generation: Testing Different Protocols to a Successful Application.
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Autorzy:
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Iborra-Egea O; MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain.
Martínez-Falguera D; MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain.
Roura S; MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain.; CIBER Cardiovascular, Instituto de Salud Carlos III, Madrid, Spain.
Bayes-Genis A; MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain.; CIBER Cardiovascular, Instituto de Salud Carlos III, Madrid, Spain.; iCor Institute, Germans Trias i Pujol University Hospital, Badalona, Spain.; Department of Medicine, Autonomous University of Barcelona, Barcelona, Spain.
Raya Á; Regenerative Medicine Program, Bellvitge Biomedical Research Institute (IDIBELL) and Program for Clinical Translation of Regenerative Medicine in Catalonia (P-CMRC), Hospital Duran i Reynals, Hospitalet de Llobregat, Barcelona, Spain.; Centre for Networked Biomedical Research on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Madrid, Spain.; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain.
Gálvez-Montón C; MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain. .; CIBER Cardiovascular, Instituto de Salud Carlos III, Madrid, Spain. .
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Źródło:
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Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2454, pp. 61-81.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
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MeSH Terms:
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Cardiovascular Diseases*
Induced Pluripotent Stem Cells*
Animals ; Cellular Reprogramming ; Fibroblasts ; Sendai virus ; Swine
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References:
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Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676. (PMID: 10.1016/j.cell.2006.07.024)
Tsang HG, Rashdan NA, Whitelaw CBA et al (2016) Large animal models of cardiovascular disease. Cell Biochem Funct 34:113–132. (PMID: 10.1002/cbf.3173)
Wu Z, Chen J, Ren J et al (2009) Generation of pig induced pluripotent stem cells with a drug-inducible system. J Mol Cell Biol 1:46–54. (PMID: 10.1093/jmcb/mjp003)
Du X, Feng T, Yu D et al (2015) Barriers for deriving transgene-free pig iPS cells with episomal vectors. Stem Cells 33:3228–3238. (PMID: 10.1002/stem.2089)
Wang G, Weng R, Lan Y et al (2017) Synergetic effects of DNA methylation and histone modification during mouse induced pluripotent stem cell generation. Sci Rep 7:39527. (PMID: 10.1038/srep39527)
Tarkowski A (1966) An air-drying method for chromosome preparations from mouse eggs. Cytogenet Genome Res 5:394–400. (PMID: 10.1159/000129914)
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Contributed Indexing:
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Keywords: Episomal nucleofection reprogramming; Porcine iPSC; RNA reprogramming; Retroviral reprogramming; Sendai virus reprogramming
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Entry Date(s):
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Date Created: 20211130 Date Completed: 20220623 Latest Revision: 20220627
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Update Code:
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20240105
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DOI:
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10.1007/7651_2021_446
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PMID:
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34845658
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Stem cell therapy has an unparalleled potential to treat blood cancers, cardiovascular diseases and neurodegenerative conditions, among others. However, stem cell therapeutics must overcome multiple requirements before reaching clinical trials, including large animal safety and efficacy studies. In cardiovascular diseases swine models are the most widely adopted due to its great translational potential to humans. In this chapter, we will describe several protocols to induce iPSC dedifferentiation in swine fibroblasts, as well as conditioning treatments that may help in the reprogramming process.
(© 2021. Springer Science+Business Media, LLC.)
