Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2.

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Tytuł:: Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2.
Autorzy:: Kwatra G; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa; Department of Clinical Microbiology, Christian Medical College, Vellore, India. Electronic address: .
Nunes M; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.
Dhar N; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.
Baillie V; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.
Serafin N; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.
Jones S; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.
Madhi SA; South African Medical Research Council: Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Department of Science and Technology/ National Research Foundation: Vaccine Preventable Diseases, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa.
Źródło:: Journal of virological methods [J Virol Methods] 2022 Feb; Vol. 300, pp. 114394. Date of Electronic Publication: 2021 Nov 30.
Typ publikacji:: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
MeSH Terms:: COVID-19*/diagnosis
Dried Blood Spot Testing*
Antibodies, Viral/*blood
Immunoglobulin G/*blood
Spike Glycoprotein, Coronavirus/*immunology
Humans ; SARS-CoV-2
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Contributed Indexing:: Keywords: Dried blood spots; Full length spike protein; Immunoglobulin (IgG); Receptor binding domain; SARS-CoV-2
Substance Nomenclature:: 0 (Antibodies, Viral)
0 (Immunoglobulin G)
0 (Spike Glycoprotein, Coronavirus)
0 (spike protein, SARS-CoV-2)
Entry Date(s):: Date Created: 20211203 Date Completed: 20220107 Latest Revision: 20230523
Update Code:: 20240105
PubMed Central ID:: PMC8631068
DOI:: 10.1016/j.jviromet.2021.114394
PMID:: 34861318
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We compared plasma and dried blood spots for detection of SARS-CoV-2 IgG antibodies. There was a good correlation between IgG values measured by both sampling methods, r = 0.935 and 0.965 for Receptor Binding Domain and full-length spike protein of SARS-CoV-2. Bland-Altman assessment showed good agreement between two sampling methods. Dried blood spots is a more pragmatic method for collecting samples for sero-epidemiological surveys of SARS-CoV-2 infection.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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