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Tytuł pozycji:

Genetic Analysis of Hexaploid Wheat ( Triticum aestivum L.) Using the Complete Sequencing of Chloroplast DNA and Haplotype Analysis of the Wknox1 Gene.

Tytuł:
Genetic Analysis of Hexaploid Wheat ( Triticum aestivum L.) Using the Complete Sequencing of Chloroplast DNA and Haplotype Analysis of the Wknox1 Gene.
Autorzy:
Gogniashvili M; Institute of Molecular Genetics, Agricultural University of Georgia, #240 D. Aghmashenebeli Av, Tbilisi 0131, Georgia.
Matsuoka Y; Graduate School of Agricultural Science, Kobe University, Rokko, Nada, Kobe 657-8501, Japan.
Beridze T; Institute of Molecular Genetics, Agricultural University of Georgia, #240 D. Aghmashenebeli Av, Tbilisi 0131, Georgia.
Źródło:
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 24; Vol. 22 (23). Date of Electronic Publication: 2021 Nov 24.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:
Gene Expression Regulation, Plant*
Genome, Chloroplast*
Haplotypes*
DNA, Chloroplast/*genetics
Homeodomain Proteins/*genetics
Plant Proteins/*genetics
Triticum/*genetics
Biological Evolution ; Polymorphism, Single Nucleotide ; Polyploidy ; Triticum/growth & development
References:
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BMC Genomics. 2012 Dec 20;13:715. (PMID: 23256920)
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Grant Information:
NFR-18-7262 Shota Rustaveli National Science Foundation
Contributed Indexing:
Keywords: Illumina; SNP; Triticum aestivum L.; Wknox1 gene; chloroplast DNA; sequencing
Substance Nomenclature:
0 (DNA, Chloroplast)
0 (Homeodomain Proteins)
0 (Knox1 protein, plant)
0 (Plant Proteins)
Entry Date(s):
Date Created: 20211210 Date Completed: 20211217 Latest Revision: 20211217
Update Code:
20240105
PubMed Central ID:
PMC8657936
DOI:
10.3390/ijms222312723
PMID:
34884525
Czasopismo naukowe
The aim of the presented study is a genetic characterization of the hexaploid wheat Triticum aestivum L. Two approaches were used for the genealogical study of hexaploid wheats-the complete sequencing of chloroplast DNA and PCR-based haplotype analysis of the fourth intron of Wknox1d and of the fifth-to-sixth-exon region of Wknox1b. The complete chloroplast DNA sequences of 13 hexaploid wheat samples were determined: Free-threshing- T. aestivum subsp. aestivum , one sample; T. aestivum subsp. compactum , two samples; T. aestivum subsp. sphaerococcum , one sample; T. aestivum subsp. carthlicoides, four samples. Hulled- T. aestivum subsp. spelta , three samples; T. aestivum subsp. vavilovii jakubz ., two samples. The comparative analysis of complete cpDNA sequences of 20 hexaploid wheat samples (13 samples in this article plus 7 samples sequenced in this laboratory in 2018) was carried out. PCR-based haplotype analysis of the fourth intron of Wknox1d and of the fifth-to-sixth exon region of Wknox1b of all 20 hexaploid wheat samples was carried out. The 20 hexaploid wheat samples (13 samples in this article plus 7 samples in 2018) can be divided into two groups- T. aestivum subsp. spelta, three samples and T. aestivum subsp. vavilovii collected in Armenia, and the remaining 16 samples, including T. aestivum subsp. vavilovii collected in Europe (Sweden). If we take the cpDNA of Chinese Spring as a reference, 25 SNPs can be identified. Furthermore, 13-14 SNPs can be identified in T. aestivum subsp. spelta and subsp. vavilovii (Vav1). In the other samples up to 11 SNPs were detected. 22 SNPs are found in the intergenic regions, 2 found in introns, and 10 SNPs were found in the genes, of which seven are synonymous. PCR-based haplotype analysis of the fourth intron of Wknox1d and the fifth-to-sixth-exon region of Wknox1b provides an opportunity to make an assumption that hexaploid wheats T. aestivum subsp. macha var. palaeocolchicum and var. letshckumicum differ from other macha samples by the absence of a 42 bp insertion in the fourth intron of Wknox1d . One possible explanation for this observation would be that two Aegilops tauschii Coss. (A) and (B) participated in the formation of hexaploids through the D genome: Ae. tauschii (A)-macha (1-5, 7, 8, 10-12), and Ae. tauschii (B)-macha M6, M9, T. aestivum subsp. aestivum cv. 'Chinese Spring' and cv. 'Red Doly'.

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