Apoptin induces pyroptosis of colorectal cancer cells via the GSDME-dependent pathway.

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Tytuł:: Apoptin induces pyroptosis of colorectal cancer cells via the GSDME-dependent pathway.
Autorzy:: Liu Z; College of Veterinary Medicine, Jilin University, Changchun, 130062, China.; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Li Y; Academician Workstation of Jilin Province, Changchun University of Chinese Medicine, Changchun, 130021, China.
Zhu Y; Academician Workstation of Jilin Province, Changchun University of Chinese Medicine, Changchun, 130021, China.
Li N; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Li W; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Shang C; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Song G; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Li S; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Cong J; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Li T; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Xiu Z; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Lu J; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Ge C; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Yang X; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Li Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.
Sun L; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.; Department of Head and Neck Surgery, Tumor Hospital of Jilin Province, Changchun, 130012, China.
Li X; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.; Academician Workstation of Jilin Province, Changchun University of Chinese Medicine, Changchun, 130021, China.; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
Jin N; College of Veterinary Medicine, Jilin University, Changchun, 130062, China.; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 130122, China.; Academician Workstation of Jilin Province, Changchun University of Chinese Medicine, Changchun, 130021, China.; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
Źródło:: International journal of biological sciences [Int J Biol Sci] 2022 Jan 01; Vol. 18 (2), pp. 717-730. Date of Electronic Publication: 2022 Jan 01 (Print Publication: 2022).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Lake Haven, N.S.W., Australia : Ivyspring International, c2004-
MeSH Terms:: Pyroptosis*
Caspase 3/*metabolism
Caspase 9/*metabolism
Colorectal Neoplasms/*metabolism
Pore Forming Cytotoxic Proteins/*metabolism
Animals ; Caspase 3/genetics ; Caspase 9/genetics ; Colorectal Neoplasms/pathology ; Cytochromes c/metabolism ; Female ; HCT116 Cells ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Mitochondria/metabolism ; Reactive Oxygen Species/metabolism ; Xenograft Model Antitumor Assays ; bcl-2-Associated X Protein/metabolism
References:: Mol Ther. 2006 Nov;14(5):637-46. (PMID: 16887394)
Nature. 2017 Jul 6;547(7661):99-103. (PMID: 28459430)
Trends Cell Biol. 2015 May;25(5):308-15. (PMID: 25639489)
CA Cancer J Clin. 2019 Jan;69(1):7-34. (PMID: 30620402)
Cell Res. 2018 Dec;28(12):1171-1185. (PMID: 30287942)
Front Oncol. 2020 Jun 25;10:1026. (PMID: 32714864)
Nat Rev Dis Primers. 2015 Nov 05;1:15065. (PMID: 27189416)
Cancer Res. 1995 Feb 1;55(3):486-9. (PMID: 7834613)
CA Cancer J Clin. 2018 Jul;68(4):250-281. (PMID: 29846947)
Int J Med Sci. 2018 Apr 3;15(7):659-665. (PMID: 29910669)
CA Cancer J Clin. 2021 May;71(3):209-249. (PMID: 33538338)
Am J Surg. 2012 Nov;204(5):741-50. (PMID: 22748294)
Biochim Biophys Acta. 2009 Aug;1793(8):1335-42. (PMID: 19374922)
Acta Pharm Sin B. 2020 Aug;10(8):1397-1413. (PMID: 32963939)
J Biol Chem. 2003 Jul 25;278(30):27729-36. (PMID: 12754198)
Nat Rev Gastroenterol Hepatol. 2019 Dec;16(12):713-732. (PMID: 31455888)
Oncogene. 2006 Apr 6;25(15):2213-22. (PMID: 16288204)
Front Cell Dev Biol. 2020 Jun 25;8:524. (PMID: 32671070)
Int J Cancer. 2019 Apr 15;144(8):1941-1953. (PMID: 30350310)
J Cell Sci. 2005 Oct 1;118(Pt 19):4485-93. (PMID: 16179607)
Cell Death Discov. 2020 Oct 28;6:112. (PMID: 33133646)
Cancers (Basel). 2019 Feb 23;11(2):. (PMID: 30813432)
J Virol. 2000 Aug;74(15):7072-8. (PMID: 10888647)
Cell Res. 2015 Dec;25(12):1285-98. (PMID: 26611636)
Immunity. 1999 Jun;10(6):629-39. (PMID: 10403638)
Mol Cancer. 2010 Jan 20;9:10. (PMID: 20085660)
Mol Ther. 2006 Nov;14(5):627-36. (PMID: 16926120)
Nature. 2015 Oct 29;526(7575):666-71. (PMID: 26375259)
Nat Rev Clin Oncol. 2017 May;14(5):297-315. (PMID: 27995949)
Cell Death Dis. 2019 Feb 25;10(3):193. (PMID: 30804337)
Drug Resist Updat. 2018 Nov;41:1-25. (PMID: 30471641)
Cell. 2014 May 22;157(5):1013-22. (PMID: 24855941)
Nature. 2015 Oct 29;526(7575):660-5. (PMID: 26375003)
Nature. 2016 Jul 7;535(7610):111-6. (PMID: 27281216)
Annu Rev Pharmacol Toxicol. 2008;48:143-69. (PMID: 17848136)
Nat Commun. 2017 Jan 03;8:14128. (PMID: 28045099)
CA Cancer J Clin. 2018 Nov;68(6):394-424. (PMID: 30207593)
BMJ. 2019 Aug 22;366:l4561. (PMID: 31439545)
Annu Rev Immunol. 2020 Apr 26;38:567-595. (PMID: 32017655)
J Natl Compr Canc Netw. 2019 May 1;17(5.5):599-601. (PMID: 31117039)
Apoptosis. 2005 Aug;10(4):717-24. (PMID: 16133863)
Cell Death Differ. 2004 May;11(5):564-73. (PMID: 14765135)
Cancers (Basel). 2020 Jun 09;12(6):. (PMID: 32526919)
Substance Nomenclature:: 0 (GSDME protein, human)
0 (Pore Forming Cytotoxic Proteins)
0 (Reactive Oxygen Species)
0 (bcl-2-Associated X Protein)
9007-43-6 (Cytochromes c)
EC 3.4.22.- (Caspase 3)
EC 3.4.22.- (Caspase 9)
Entry Date(s):: Date Created: 20220110 Date Completed: 20220321 Latest Revision: 20220322
Update Code:: 20240105
PubMed Central ID:: PMC8741846
DOI:: 10.7150/ijbs.64350
PMID:: 35002520
: Czasopismo naukowe

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Apoptin is a small molecular weight protein encoded by the VP3 gene of chicken anemia virus (CAV). It can induce apoptosis of tumor cells and play anti-tumorigenic functions. In this study, we identified a time-dependent inhibitory role of apoptin on the viability of HCT116 cells. We also demonstrated that apoptin induces pyroptosis through cleaved caspase 3, and with a concomitant cleavage of gasdermin E (GSDME) rather than GSDMD. GSDME knockdown switched the apoptin-induced cell death from pyroptosis to apoptosis in vitro . Furthermore, we demonstrated that the effect of apoptin on GSDME-dependent pyroptosis could be mitigated by caspase-3 and caspase-9 siRNA knockdown. Additionally, apoptin enhanced the intracellular reactive oxygen species (ROS), causing aggregation of the mitochondrial membrane protein Tom20. Moreover, bax and cytochrome c were released to the activating caspase-9, eventually triggering pyroptosis. Therefore, GSDME mediates the apoptin-induced pyroptosis through the mitochondrial apoptotic pathway. Finally, using nude mice xenografted with HCT116 cells, we found that apoptin induces pyroptosis and significantly inhibits tumor growth. Based on this mechanism, apoptin may provide a new strategy for colorectal cancer therapy.
Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
(© The author(s).)

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