A novel molecular diagnostic method for the gut content analysis of Philaenus DNA.

Tytuł:: A novel molecular diagnostic method for the gut content analysis of Philaenus DNA.
Autorzy:: Rodrigues I; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal.; Departamento de Ingeniería Agrária, Universidad de Léon, Av. Portugal, n° 41, 24071, Léon, Spain.
Ramos V; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal.
Benhadi-Marín J; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal.
Moreno A; Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas (ICA-CSIC), C/Serrano 115 dpdo, 28006, Madrid, Spain.
Fereres A; Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas (ICA-CSIC), C/Serrano 115 dpdo, 28006, Madrid, Spain.
Pereira JA; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal.
Baptista P; Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal. .
Źródło:: Scientific reports [Sci Rep] 2022 Jan 11; Vol. 12 (1), pp. 492. Date of Electronic Publication: 2022 Jan 11.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: DNA/*genetics
Hemiptera/*genetics
Insect Vectors/*genetics
Spiders/*physiology
Animals ; DNA Primers/genetics ; Feeding Behavior ; Gastrointestinal Tract/metabolism ; Hemiptera/metabolism ; Insect Vectors/metabolism ; Pest Control, Biological ; Polymerase Chain Reaction ; Predatory Behavior
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Grant Information:: 2020.07051.BD Fundação para a Ciência e a Tecnologia; UIDB/00690/2020 Fundação para a Ciência e a Tecnologia; UIDB/00690/2020 Fundação para a Ciência e a Tecnologia; UIDB/00690/2020 Fundação para a Ciência e a Tecnologia; UIDB/00690/2020 Fundação para a Ciência e a Tecnologia; 727987 Horizon 2020 Framework Programme; 727987 Horizon 2020 Framework Programme; 727987 Horizon 2020 Framework Programme; 727987 Horizon 2020 Framework Programme
Substance Nomenclature:: 0 (DNA Primers)
9007-49-2 (DNA)
Entry Date(s):: Date Created: 20220112 Date Completed: 20220228 Latest Revision: 20220228
Update Code:: 20240104
PubMed Central ID:: PMC8752687
DOI:: 10.1038/s41598-021-04422-1
PMID:: 35017549
: Czasopismo naukowe

Pełny tekst

Philaenus spumarius is a vector of Xylella fastidiosa, one of the most dangerous plants pathogenic bacteria worldwide. There is currently no control measure against this pathogen. Thus, the development of vector control strategies, like generalist predators, such as spiders, could be essential to limit the spread of this vector-borne pathogen. In this study, a polymerase chain reaction (PCR)-based approach was developed to principally detect DNA of P. spumarius in the spider's gut. Accordingly, 20 primer pairs, targeting the mitochondrial cytochrome oxidase I (COI) and cytochrome b (cytB) genes, were tested for specificity, sensitivity, and efficiency in detecting P. spumarius DNA. Overall, two primer sets, targeting COI gene (COI_Ph71F/COI_Ph941R) and the cytB gene (cytB_Ph85F/cytB_Ph635R), showed the highest specificity and sensitivity, being able to amplify 870 pb and 550 bp fragments, respectively, with P. spumarius DNA concentrations 100-fold lower than that of the DNA of non-target species. Among these two primer sets, the cytB_Ph85F/cytB_Ph635R was able to detect P. spumarius in the spider Xysticus acerbus, reaching 50% detection success 82 h after feeding. The feasibility of this primer set to detect predation of P. spumarius by spiders was confirmed in the field, where 20% of the collected spiders presented positive amplifications.
(© 2022. The Author(s).)

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