A modular RNA interference system for multiplexed gene regulation.

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Abstrakt

Tytuł:: A modular RNA interference system for multiplexed gene regulation.
Autorzy:: Dwijayanti A
Storch M; Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
Stan GB; Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.; Department of Bioengineering, Bessemer Building, South Kensington Campus, Imperial College London, London SW7 2AZ, UK.
Baldwin GS; Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.; Department of Life Sciences, Sir Alexander Fleming Building, South Kensington Campus, Imperial College London, London SW7 2AZ, UK.
Źródło:: Nucleic acids research [Nucleic Acids Res] 2022 Feb 22; Vol. 50 (3), pp. 1783-1793.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Metabolic Engineering*
Gene Expression Regulation ; Gene Regulatory Networks ; Genetic Engineering ; RNA Interference
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Entry Date(s):: Date Created: 20220121 Date Completed: 20220415 Latest Revision: 20220415
Update Code:: 20240105
PubMed Central ID:: PMC8860615
DOI:: 10.1093/nar/gkab1301
PMID:: 35061908
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The rational design and realisation of simple-to-use genetic control elements that are modular, orthogonal and robust is essential to the construction of predictable and reliable biological systems of increasing complexity. To this effect, we introduce modular Artificial RNA interference (mARi), a rational, modular and extensible design framework that enables robust, portable and multiplexed post-transcriptional regulation of gene expression in Escherichia coli. The regulatory function of mARi was characterised in a range of relevant genetic contexts, demonstrating its independence from other genetic control elements and the gene of interest, and providing new insight into the design rules of RNA based regulation in E. coli, while a range of cellular contexts also demonstrated it to be independent of growth-phase and strain type. Importantly, the extensibility and orthogonality of mARi enables the simultaneous post-transcriptional regulation of multi-gene systems as both single-gene cassettes and poly-cistronic operons. To facilitate adoption, mARi was designed to be directly integrated into the modular BASIC DNA assembly framework. We anticipate that mARi-based genetic control within an extensible DNA assembly framework will facilitate metabolic engineering, layered genetic control, and advanced genetic circuit applications.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)

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