CircCBFB is a mediator of hepatocellular carcinoma cell autophagy and proliferation through miR-424-5p/ATG14 axis.

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Tytuł:: CircCBFB is a mediator of hepatocellular carcinoma cell autophagy and proliferation through miR-424-5p/ATG14 axis.
Autorzy:: Zhao Z; Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, No. 138, Tongzipo Road, Yuelu District, Changsha, Hunan, 410013, People's Republic of China.
He J; Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, No. 138, Tongzipo Road, Yuelu District, Changsha, Hunan, 410013, People's Republic of China.
Feng C; Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, No. 138, Tongzipo Road, Yuelu District, Changsha, Hunan, 410013, People's Republic of China. .
Źródło:: Immunologic research [Immunol Res] 2022 Jun; Vol. 70 (3), pp. 341-353. Date of Electronic Publication: 2022 Jan 23.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Totawa Nj : Humana Press
Original Publication: Basel ; New York : S. Karger, [c1986-
MeSH Terms:: Adaptor Proteins, Vesicular Transport*/genetics
Adaptor Proteins, Vesicular Transport*/metabolism
Autophagy-Related Proteins*/genetics
Autophagy-Related Proteins*/metabolism
Carcinoma, Hepatocellular*/pathology
Liver Neoplasms*/pathology
MicroRNAs*/genetics
Apoptosis/genetics ; Autophagy/genetics ; Beclin-1/genetics ; Beclin-1/metabolism ; Cell Line ; Cell Line, Tumor ; Cell Proliferation/genetics ; Gene Expression Regulation, Neoplastic ; Humans ; In Situ Hybridization, Fluorescence ; RNA, Circular/genetics
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Contributed Indexing:: Keywords: ATG14; Autophagy; CircCBFB, miR-424-5p; Hepatocellular carcinoma
Substance Nomenclature:: 0 (ATG14 protein, human)
0 (Adaptor Proteins, Vesicular Transport)
0 (Autophagy-Related Proteins)
0 (Beclin-1)
0 (MIRN424 microrna, human)
0 (MicroRNAs)
0 (RNA, Circular)
Entry Date(s):: Date Created: 20220123 Date Completed: 20220616 Latest Revision: 20221109
Update Code:: 20240105
DOI:: 10.1007/s12026-021-09255-8
PMID:: 35066780
: Czasopismo naukowe

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This study aims to investigate the role of circCBFB in hepatocellular carcinoma (HCC) cell proliferation and autophagy. qRT-PCR and Western blotting analyses quantified the expression levels of circCBFB, miR-424-5p, and ATG14 in HCC tissues and/or HCC cell lines. After transfection with pcDNA3.1-CircCBFB, sh-CircCBFB, miR-424-5p mimic, miR-424-5p inhibitor, pcDNA3.1-ATG14, sh-ATG14, sh-CircCBFB + miR-424-5p inhibitor, pcDNA3.1-CircCBFB + miR-424-5p mimic, sh-CircCBFB + pcDNA3.1-ATG14, or pcDNA3.1-CircCBFB + sh-ATG14, the proliferation, cell cycle, and apoptosis of Huh-7 and HCCLM3 cells were detected, respectively, through MTT assay and flow cytometry. Western blotting measured the expression levels of ATG14 and autophagy-related proteins (LC3-ΙΙ/LC3-Ι, Beclin1, and p62). The interactions among circCBFB, miR-424-5p, and ATG14 were identified through RNA fluorescence in situ hybridization and RNA immunoprecipitation. In HCC tissues, circCBFB and ATG14 were highly expressed, and miR-424-5p expression was downregulated. Transfection of pcDNA3.1-CircCBFB, miR-424-5p inhibitor, or pcDNA3.1-ATG14 into HCC cells facilitated HCC cell proliferation and autophagy, while suppressing cell apoptosis, evidenced by elevated cell viability, increased protein levels of autophagosome markers (LC3-ΙΙ/LC3-Ι and Beclin1), repressed apoptosis rate, and suppressed protein level of autophagy receptor p62. miR-424-5p was a target gene of circCBFB, and miR-424-5p negatively mediated ATG14. CircCBFB inhibits miR-424-5p and upregulates ATG14, thus promoting HCC cell proliferation and autophagy.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

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