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Tytuł:
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Autophagy-independent cytoprotection by optineurin from toxicity of aggregates formed by mutant huntingtin and mutant ataxin-3.
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Autorzy:
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Moharir SC; CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, India.; Tata Institute for Genetics and Society, Bangalore 560065, India.
Raghawan AK; CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Ramaswamy R; CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Swarup G; CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
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Źródło:
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Journal of biochemistry [J Biochem] 2022 May 11; Vol. 171 (5), pp. 555-565.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: : Abingdon, UK : Oxford University Press
Original Publication: Tokyo : Japanese Biochemical Society
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MeSH Terms:
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Cytoprotection*
Protein Aggregates*
Animals ; Ataxin-3/genetics ; Autophagy/physiology ; Fibroblasts ; Glutamine ; Mice ; Mutant Proteins
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Contributed Indexing:
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Keywords: autophagy; mutant ataxin-3; mutant huntingtin; mutant protein aggregates; neurodegeneration; optineurin
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Substance Nomenclature:
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0 (Mutant Proteins)
0 (Protein Aggregates)
0RH81L854J (Glutamine)
EC 3.4.19.12 (Ataxin-3)
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Entry Date(s):
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Date Created: 20220208 Date Completed: 20220519 Latest Revision: 20220519
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Update Code:
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20240104
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DOI:
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10.1093/jb/mvac011
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PMID:
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35134975
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An important feature of several neurodegenerative diseases is the formation of pathological structures containing aggregated proteins. The autophagy receptor optineurin/OPTN is frequently observed in these structures. The role played by optineurin in these aggregates is not clear. In this study, we explored whether optineurin has a cytoprotective role in the cells having mutant protein aggregates. We overexpressed mutant huntingtin having 97 glutamine repeats (mHtt) and mutant ataxin-3 having 130 glutamine repeats (mAtax-3) in wild-type and optineurin-deficient neuronal (N2A) and non-neuronal cells (Optn-/- mouse embryonic fibroblasts) and determined the percentage of dead cells with mutant protein aggregates. Optineurin-deficient cells having mHtt or mAtax-3 aggregates showed higher cell death as compared to wild-type cells having mutant protein aggregates. Confocal microscopy revealed that optineurin formed a shell around mHtt and mAtax-3 aggregates through its C-terminal domain. The C-terminal domain of optineurin, which lacks LC3-interacting region required for autophagy, was necessary and sufficient to reduce cytotoxicity of mHtt and mAtax-3 aggregates. Our results show that in the absence of optineurin, mutant protein aggregates are highly toxic, revealing an autophagy-independent cytoprotective function of optineurin, which is mediated by its C-terminal domain.
(© The Author(s) 2022. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.)
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