Versioning biological cells for trustworthy cell engineering.

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Abstrakt

Tytuł:: Versioning biological cells for trustworthy cell engineering.
Autorzy:: Tellechea-Luzardo J; Interdisciplinary Computing and Complex Biosystems (ICOS) Research Group, Newcastle University, Newcastle Upon Tyne, NE4 5TG, UK.
Hobbs L; Interdisciplinary Computing and Complex Biosystems (ICOS) Research Group, Newcastle University, Newcastle Upon Tyne, NE4 5TG, UK.
Velázquez E; Systems and Synthetic Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049, Madrid, Spain.
Pelechova L; Interdisciplinary Computing and Complex Biosystems (ICOS) Research Group, Newcastle University, Newcastle Upon Tyne, NE4 5TG, UK.
Woods S; Policy Ethics and Life Sciences (PEALS), Newcastle University, Newcastle Upon Tyne, NE1 7RU, UK.
de Lorenzo V; Systems and Synthetic Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049, Madrid, Spain.
Krasnogor N; Interdisciplinary Computing and Complex Biosystems (ICOS) Research Group, Newcastle University, Newcastle Upon Tyne, NE4 5TG, UK. .
Źródło:: Nature communications [Nat Commun] 2022 Feb 09; Vol. 13 (1), pp. 765. Date of Electronic Publication: 2022 Feb 09.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [London] : Nature Pub. Group
MeSH Terms:: Biological Products*
Cell Engineering/*methods
Animals ; Automation ; Bacteria/genetics ; Bacteria/metabolism ; Biotechnology ; Cell Line ; Genetic Engineering/methods ; Humans ; Metabolic Engineering ; Synthetic Biology/methods
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Substance Nomenclature:: 0 (Biological Products)
Entry Date(s):: Date Created: 20220210 Date Completed: 20220307 Latest Revision: 20221022
Update Code:: 20240104
PubMed Central ID:: PMC8828774
DOI:: 10.1038/s41467-022-28350-4
PMID:: 35140226
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"Full-stack" biotechnology platforms for cell line (re)programming are on the horizon, thanks mostly to (a) advances in gene synthesis and editing techniques as well as (b) the growing integration of life science research with informatics, the internet of things and automation. These emerging platforms will accelerate the production and consumption of biological products. Hence, traceability, transparency, and-ultimately-trustworthiness is required from cradle to grave for engineered cell lines and their engineering processes. Here we report a cloud-based version control system for biotechnology that (a) keeps track and organizes the digital data produced during cell engineering and (b) molecularly links that data to the associated living samples. Barcoding protocols, based on standard genetic engineering methods, to molecularly link to the cloud-based version control system six species, including gram-negative and gram-positive bacteria as well as eukaryote cells, are shown. We argue that version control for cell engineering marks a significant step toward more open, reproducible, easier to trace and share, and more trustworthy engineering biology.
(© 2022. The Author(s).)

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