-
Tytuł:
-
Anti-inflammatory effect of flavonoids from chestnut flowers in lipopolysaccharide-stimulated RAW 264.7 macrophages and acute lung injury in mice.
-
Autorzy:
-
Peng F; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China. Electronic address: .
Yin H; Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China. Electronic address: .
Du B; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China. Electronic address: .
Niu K; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China. Electronic address: .
Yang Y; Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China; Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China. Electronic address: .
Wang S; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin, 300457, China. Electronic address: .
-
Źródło:
-
Journal of ethnopharmacology [J Ethnopharmacol] 2022 May 23; Vol. 290, pp. 115086. Date of Electronic Publication: 2022 Feb 12.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
-
MeSH Terms:
-
Acute Lung Injury/*pathology
Anti-Inflammatory Agents/*pharmacology
Flavonoids/*pharmacology
Plant Extracts/*pharmacology
Animals ; Bronchoalveolar Lavage Fluid/cytology ; Cell Survival/drug effects ; Chromatography, High Pressure Liquid ; Cytokines/drug effects ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Flowers ; Glutathione/drug effects ; Inflammation Mediators/metabolism ; Lipopolysaccharides/pharmacology ; Lung/drug effects ; Macrophages/drug effects ; Male ; Malondialdehyde/metabolism ; Mice ; Mice, Inbred BALB C ; Nitric Oxide Synthase Type II/drug effects ; Oxidative Stress/drug effects ; RAW 264.7 Cells ; Random Allocation ; Superoxide Dismutase/drug effects ; Tandem Mass Spectrometry
-
Contributed Indexing:
-
Keywords: Acute lung injury; Anti-inflammatory; Chestnut flower; Flavonoids; Macrophages
-
Substance Nomenclature:
-
0 (Anti-Inflammatory Agents)
0 (Cytokines)
0 (Flavonoids)
0 (Inflammation Mediators)
0 (Lipopolysaccharides)
0 (Plant Extracts)
4Y8F71G49Q (Malondialdehyde)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.15.1.1 (Superoxide Dismutase)
GAN16C9B8O (Glutathione)
-
Entry Date(s):
-
Date Created: 20220214 Date Completed: 20220323 Latest Revision: 20220323
-
Update Code:
-
20240105
-
DOI:
-
10.1016/j.jep.2022.115086
-
PMID:
-
35157952
-
Ethnopharmacological Relevance: Chestnut flowers were one of the by-products during chestnut industrial processing. Chestnut (Castanea mollissima Blume) flower is rich in flavonoids and has been used as a traditional medicine to treat a variety of diseases including respiratory disorders for a long history.
Aim of the Study: The present study aims to investigate the potential anti-inflammatory effect of flavonoids from chestnut flower (FCF) in lipopolysaccharide (LPS)-treated RAW 264.7 cells and stimulated acute lung injury (ALI) in mice.
Materials and Methods: HPLC-ESI-MS/MS was applied to identify flavonoids from Chestnut flower. The ROS content in cells and lung tissue was measured by flow cytometry. The malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and glutathione (GSH) content in cells and bronchoalveolar lavage fluid (BALF) was analyzed by photometry. Furthermore, the level of pro-inflammatory factors was analyzed by ELISA, and the expression of inflammatory gene mRNA by fluorescence quantitative PCR. H&E staining was used to evaluate the degree of lung tissue injury in mice. MPO activity was used to measure the degree of neutrophil infiltration. Total protein content was detected by BCA method.
Results: A total of forty-nine flavonoids compounds were tentatively identified in FCF by mass spectrometry analysis. The results of cell experiment suggested that FCF could alleviate oxidative injury via increasing SOD activity and GSH content, as well as inhibiting the production of intracellular ROS and MDA. FCF exerted its protective effect by suppressing the expression of both inducible nitric oxide synthase (iNOS) and cycooxygenase 2 (COX-2) to inhibit the synthesis of pro-inflammatory factors and cytokines, including NO, PGE2, TNF-α, IL-6 and IL-1β. Besides, FCF treatment could alleviate the thickening of alveolar wall and pulmonary congestion in LPS-treated ALI mice, and significantly inhibit the activity of myeloperoxidas (MPO) and the expression of cytokines in BALF.
Conclusions: FCF could ameliorate inflammation and oxidative stress in LPS-treated inflammation, resulting in an overall improvement in both macroscopic and histological parameters.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
