MTHFR 677TT is associated with decreased number of embryos and cumulative live birth rate in patients undergoing GnRHa short protocol: a retrospective study.

Tytuł:: MTHFR 677TT is associated with decreased number of embryos and cumulative live birth rate in patients undergoing GnRHa short protocol: a retrospective study.
Autorzy:: Zeng H; Department of Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.; Department of Reproductive Medicine Center, Foshan Maternal and Child Health Care Hospital, Southern Medical University, Foshan, 528000, Guangdong, China.; Department of Reproductive Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, China.
Liu Z; Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, Guangdong, China.
Zhang L; Department of Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Liu N; Department of Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China. dr_.
Źródło:: BMC pregnancy and childbirth [BMC Pregnancy Childbirth] 2022 Mar 01; Vol. 22 (1), pp. 170. Date of Electronic Publication: 2022 Mar 01.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, [2001-
MeSH Terms:: Genotype*
Reproductive Techniques, Assisted*
Gonadotropin-Releasing Hormone/*agonists
Methylenetetrahydrofolate Reductase (NADPH2)/*genetics
Ovulation Induction/*methods
Adult ; Embryo Transfer/methods ; Female ; Fertilization in Vitro ; Humans ; Live Birth/genetics ; Pregnancy ; Pregnancy Outcome/genetics ; Pregnancy Rate ; Regression Analysis ; Retrospective Studies
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Grant Information:: 81571441 the National Natural Science Foundation of China
Contributed Indexing:: Keywords: 5,10-methylenetetrahydrofolate reductase; Cumulative live birth rate; Retrospective analysis
Substance Nomenclature:: 33515-09-2 (Gonadotropin-Releasing Hormone)
EC 1.5.1.20 (MTHFR protein, human)
EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
Entry Date(s):: Date Created: 20220302 Date Completed: 20220316 Latest Revision: 20220318
Update Code:: 20240104
PubMed Central ID:: PMC8887009
DOI:: 10.1186/s12884-022-04506-4
PMID:: 35232413
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Pełny tekst

Background: Whether MTHFR C677T genotype affects pregnancy outcomes following assisted reproductive technology is conflicting. And the role of MTHFR C677T genotype on cumulative live birth has not been reported. This study aims to investigate the effect of MTHFR C677T genotype on cumulative live birth following in-vitro fertilization and embryo transfer (IVF-ET).
Methods: This is a retrospective cohort study that includes 1173 women undergoing their first IVF-ET. We retrospectively compared the reproductive outcomes among the groups stratified by MTHFR C677T genotypes (677CC, 677CT, 677TT). We performed interaction analysis to detect the factor that interacts with the MTHFR C677T genotype. Poisson regression analyses were used to evaluate the associations between MTHFR C677T genotypes with the number of transferable embryos and the number of good-quality embryos. Cox regression analysis was used to evaluate the association between MTHFR C677T genotypes with cumulative live birth. All regression analyses were adjusted with the confounding factors which may independently impact reproductive outcomes.
Results: There is a significant interactive effect of MTHFR 677TT genotype with GnRHa protocol on reproductive outcomes (P for interaction<0.05). MTHFR 677TT homozygous mutation was found to impact reproductive outcomes under GnRHa short protocol but not GnRHa long protocol. MTHFR 677TT is significantly associated with decreased number of transferable embryos (p-value=0.028), decreased number of good-quality embryos (p-value=0.005), and decreased cumulative live birth rate (p-value=0.024) in patients undergoing GnRHa short protocol. However, the clinical pregnancy rate, miscarriage rate and live birth rate at the first embryo transfer cycle were not significantly different between the groups under both protocols (p-values>0.05).
Conclusions: MTHFR 677TT genotype is associated with decreased number of transferable embryos, decreased number of good-quality embryos, and decreased cumulative live birth rate in the first complete cycle in patients undergoing GnRHa short protocol.
(© 2022. The Author(s).)

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