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Tytuł:
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Use of Cotton Balls in Diapers for Collection of Urine Samples Impacts the Analysis of Routine Chemistry Tests: An Evaluation of Cotton Balls, Diapers, and Chemistry Analyzers.
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Autorzy:
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Thomas SN; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN.
Stieglitz HM; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH. Electronic address: .
Hackenmueller S; Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI.
Suh-Lailam B; Department of Pathology and Laboratory Medicine, Ann and Robert H. Lurie Children's Hospital of Chicago, Chicago, IL; Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL.
Pyle-Eilola AL; Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH; Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, OH.
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Źródło:
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The Journal of pediatrics [J Pediatr] 2022 Jun; Vol. 245, pp. 179-183.e8. Date of Electronic Publication: 2022 Mar 03.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: St. Louis, MO : Mosby
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MeSH Terms:
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Cotton Fiber*
Specimen Handling*/instrumentation
Urinalysis*/methods
Albumins ; Diapers, Infant ; Humans ; Phosphorus ; Sodium ; Urea
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Contributed Indexing:
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Keywords: chemistry; cotton ball; diaper; laboratory testing; pediatrics; urine collection
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Substance Nomenclature:
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0 (Albumins)
27YLU75U4W (Phosphorus)
8W8T17847W (Urea)
9NEZ333N27 (Sodium)
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Entry Date(s):
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Date Created: 20220306 Date Completed: 20220621 Latest Revision: 20230614
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Update Code:
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20240105
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DOI:
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10.1016/j.jpeds.2022.02.051
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PMID:
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35248569
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Objective: To evaluate the suitability of urine samples collected with cotton balls placed into diapers for routine laboratory chemistry analyses.
Study Design: Twenty pools of residual unpreserved urine samples were separated into control and treated aliquots. The treated samples were absorbed into 2 different brands of cotton balls, wrapped in 3 different brands of diapers, and incubated at 37°C for 1 hour. The urine-soaked cotton balls were placed into a syringe and expressed via plunger depression. Urine sodium, potassium, creatinine, urea, calcium, magnesium, inorganic phosphorus, albumin, and total protein were measured on all samples on 5 automated clinical chemistry platforms: Ortho Vitros 4600, Siemens Dimension Vista 500, Beckman Coulter AU5822, Roche Cobas 6000, and Abbott Architect c8000 at 5 separate hospital laboratories. Criteria used to exclude the presence of significant effects of urine from presoaked cotton balls in a diaper on the measurement of chemistry laboratory tests were R 2 >0.95, slope of 0.9-1.1, and mean bias within ±10%.
Results: Albumin and total protein measurements demonstrated significant negative bias in urine from both brands of presoaked cotton balls with all brands of diapers on all 5 chemistry platforms compared with the control urine. We did not observe a significant effect of presoaking urine in cotton balls in a diaper on the measurement of sodium, inorganic phosphorus, and urea. The remaining tests demonstrated significant effects when measured in urine from presoaked cotton balls and/or diapers that were specific to the chemistry analyzer platform or diaper.
Conclusions: Diaper and cotton ball-based urine collection significantly impacts the measurement of several common chemistry assays.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Comment in: J Pediatr. 2023 Apr;255:259. (PMID: 36402432)
