Calcium-sensing receptor is not expressed in the absorptive enterocytes of weaned piglets.

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Abstrakt

Tytuł:: Calcium-sensing receptor is not expressed in the absorptive enterocytes of weaned piglets.
Autorzy:: Zhao X; Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Hui Q; Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Azevedo P; Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Nyachoti CM; Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
O K; Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.; CCARM, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada.
Yang C; Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Źródło:: Journal of animal science [J Anim Sci] 2022 Apr 01; Vol. 100 (4).
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Champaign, IL : American Society of Animal Science
MeSH Terms:: Enterocytes*
Receptors, Calcium-Sensing*/genetics
Receptors, Calcium-Sensing*/metabolism
Animals ; Epithelial Cells/metabolism ; Intestinal Mucosa/metabolism ; Intestines ; Swine ; Weaning
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Grant Information:: RGPIN-2017-04967 Natural Sciences and Engineering Council of Canada; Canada Foundation for Innovation; University of Manitoba
Contributed Indexing:: Keywords: calcium-sensing receptor; enterocytes; enteroendocrine cells; weaned piglets
Local Abstract: [plain-language-summary] The calcium-sensing receptor (CaSR) has been identified as a nutrient sensor along the gastrointestinal tract and plays a pivotal role in food digestion, nutrient absorption, and energy metabolism. Hence, elucidating the expression pattern of pig CaSR (pCaSR) in the intestinal segments, especially in the absorptive enterocytes, could reveal the significance of pCaSR in nutrient sensing in the gastrointestinal tract. In the present work, after isolating enterocytes from the ileum of weaned piglets, we investigated the expression of pCaSR in porcine enterocytes through a combination of biological tools at both gene and protein expression levels. Data observed in the current study revealed that porcine enterocytes could be obtained by using cell sorting with a digestive enzyme (sucrase-isomaltase) as the enterocyte-specific marker. Plus, the pCaSR is not expressed in either isolated porcine ileal enterocytes or the intestinal porcine enterocyte cell line-j2 (IPEC-J2).
Substance Nomenclature:: 0 (Receptors, Calcium-Sensing)
Entry Date(s):: Date Created: 20220316 Date Completed: 20220411 Latest Revision: 20230317
Update Code:: 20240105
PubMed Central ID:: PMC9030235
DOI:: 10.1093/jas/skac085
PMID:: 35294536
: Czasopismo naukowe

The calcium-sensing receptor (CaSR) is a kokumi receptor that plays an essential role in nutrient sensing and animal physiology, growth, and development. Pig CaSR (pCaSR) was identified and characterized in the intestine. However, further research is still needed to confirm the expression of CaSR in the epithelial cells isolated from weaned piglets. In this study, primary enterocytes were isolated and characterized from the ileum of weaned piglets by the Weiser distended intestinal sac technique and fluorescence-activated cell sorting (FACS) based on sucrase-isomaltase (SI) as an enterocyte-specific marker. The expression of CaSR was investigated in both primary enterocytes and the intestinal porcine enterocyte cell line-j2 (IPEC-J2) by droplet digital polymerase chain reaction (ddPCR), immunofluorescence staining, and Western blotting. Results demonstrated that porcine enterocytes could be obtained using FACS with the SI as the enterocyte-specific marker and that pCaSR is not expressed in both porcine ileal enterocytes and IPEC-J2 cells, which specifically identified the expression of pCaSR in ileal enterocytes with sensitive and specific approaches.
(© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.  For permissions, please e-mail: journals.permissions@oup.com.)

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