MDC1 is essential for G2/M transition and spindle assembly in mouse oocytes.

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Abstrakt

Tytuł:: MDC1 is essential for G2/M transition and spindle assembly in mouse oocytes.
Autorzy:: Leem J; Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, Korea.
Oh JS; Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, Korea. .; Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, Korea. .
Źródło:: Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2022 Mar 23; Vol. 79 (4), pp. 200. Date of Electronic Publication: 2022 Mar 23.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Basel : Springer
Original Publication: Basel ; Boston : Birkhauser, c1997-
MeSH Terms:: Cell Cycle Proteins*/metabolism
Oocytes*/metabolism
Animals ; Cell Cycle ; Cell Division ; DNA Damage ; Mammals/metabolism ; Mice
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Grant Information:: NRF-2017R1A6A1A03015642 Basic Science Research Program through the National Research Foundation of Korea (NRF); NRF-2019R1I1A2A01041413 Basic Science Research Program through the National Research Foundation of Korea (NRF)
Contributed Indexing:: Keywords: APC/C-Cdh1; DNA damage; G2/M transition; MDC1; Oocytes; Spindle assembly
Substance Nomenclature:: 0 (Cell Cycle Proteins)
Entry Date(s):: Date Created: 20220323 Date Completed: 20220404 Latest Revision: 20220405
Update Code:: 20240104
DOI:: 10.1007/s00018-022-04241-1
PMID:: 35320416
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Mammalian oocytes are particularly susceptible to accumulating DNA damage. However, unlike mitotic cells in which DNA damage induces G2 arrest by activating the ATM-Chk1/2-Cdc25 pathway, oocytes readily enter M-phase immediately following DNA damage. This implies a lack of a robust canonical G2/M DNA damage checkpoint in oocytes. Here we show that MDC1 plays a non-canonical role in controlling G2/M transition by regulating APC/C-Cdh1-mediated cyclin B1 degradation in response to DNA damage in mouse oocytes. Depletion of MDC1 impaired M-phase entry by decreasing cyclin B1 levels via the APC/C-Cdh1 pathway. Notably, the APC/C-Cdh1 regulation mediated by MDC1 was achieved by a direct interaction between MDC1 and APC/C-Cdh1. This interaction was transiently disrupted after DNA damage with a concomitant increase in Cdh1 levels, which, in turn, decreased cyclin B1 levels and delayed M-phase entry. Moreover, MDC1 depletion impaired spindle assembly by decreasing the integrity of microtubule organizing centers (MTOCs). Therefore, our results demonstrate that MDC1 is an essential molecule in regulating G2/M transition in response to DNA damage and in regulating spindle assembly in mouse oocytes. These results provide new insights into the regulation of the G2/M DNA damage checkpoint and cell cycle control in oocytes.
(© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

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