Structural and Molecular Characterization of Squalene Synthase Belonging to the Marine Thraustochytrid Species Aurantiochytrium limacinum Using Bioinformatics Approach.

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Tytuł:: Structural and Molecular Characterization of Squalene Synthase Belonging to the Marine Thraustochytrid Species Aurantiochytrium limacinum Using Bioinformatics Approach.
Autorzy:: Vyas S; Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resource Engineering, Luleå University of Technology, 97187 Luleå, Sweden.
Bettiga M; Department of Biological Engineering, Chalmers University of Technology, 41296 Gothenberg, Sweden.; Bioeconomy Division, EviKrets Biobased Processes Consultants, Lunnavågen 87, 42834 Landvetter, Sweden.
Rova U; Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resource Engineering, Luleå University of Technology, 97187 Luleå, Sweden.
Christakopoulos P; Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resource Engineering, Luleå University of Technology, 97187 Luleå, Sweden.
Matsakas L; Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resource Engineering, Luleå University of Technology, 97187 Luleå, Sweden.
Patel A; Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resource Engineering, Luleå University of Technology, 97187 Luleå, Sweden.
Źródło:: Marine drugs [Mar Drugs] 2022 Feb 28; Vol. 20 (3). Date of Electronic Publication: 2022 Feb 28.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2003]-
MeSH Terms:: Aquatic Organisms/*enzymology
Farnesyl-Diphosphate Farnesyltransferase/*chemistry
Farnesyl-Diphosphate Farnesyltransferase/*genetics
Stramenopiles/*enzymology
Amino Acid Sequence ; Aquatic Organisms/genetics ; Binding Sites ; Computational Biology ; Ligands ; Models, Molecular ; Molecular Structure ; Stramenopiles/genetics
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Grant Information:: 2020-01028 Swedish Research Council; JCK-2115 L3 Kempe Foundation
Contributed Indexing:: Keywords: Aurantiochytrium; bioinformatic analysis; marine thraustochytrids; squalene; squalene synthase
Substance Nomenclature:: 0 (Ligands)
EC 2.5.1.21 (Farnesyl-Diphosphate Farnesyltransferase)
Entry Date(s):: Date Created: 20220324 Date Completed: 20220331 Latest Revision: 20220331
Update Code:: 20240104
PubMed Central ID:: PMC8955342
DOI:: 10.3390/md20030180
PMID:: 35323479
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The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the tri-terpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites.

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