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Tytuł:
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Protective effect of total Saponins from American ginseng against cigarette smoke-induced COPD in mice based on integrated metabolomics and network pharmacology.
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Autorzy:
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Lin H; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Wang C; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Yu H; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Liu Y; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Tan L; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
He S; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Li Z; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Wang C; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Research Center of Natural Drug, Jilin University, Changchun 130021, China.
Wang F; College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Li P; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Research Center of Natural Drug, Jilin University, Changchun 130021, China.
Liu J; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Research Center of Natural Drug, Jilin University, Changchun 130021, China. Electronic address: .
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Źródło:
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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2022 May; Vol. 149, pp. 112823. Date of Electronic Publication: 2022 Mar 23.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Paris : Editions Scientifiques Elsevier
Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
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MeSH Terms:
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Cigarette Smoking*
Panax*/chemistry
Pulmonary Disease, Chronic Obstructive*/drug therapy
Pulmonary Disease, Chronic Obstructive*/prevention & control
Saponins*/metabolism
Saponins*/pharmacology
Saponins*/therapeutic use
Animals ; Biomarkers/metabolism ; Metabolomics/methods ; Mice ; Molecular Docking Simulation ; Network Pharmacology
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Contributed Indexing:
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Keywords: American ginseng; Chronic obstructive pulmonary disease; Metabolomics; Network pharmacology; Total saponins
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Substance Nomenclature:
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0 (Biomarkers)
0 (Saponins)
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Entry Date(s):
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Date Created: 20220325 Date Completed: 20220427 Latest Revision: 20220427
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Update Code:
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20240105
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DOI:
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10.1016/j.biopha.2022.112823
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PMID:
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35334426
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Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory disease. Aiming at assessing the effect of total saponins from American ginseng on COPD, both the chemical composition and anti-COPD activity of total saponins from wild-simulated American ginseng (TSW) and field-grown American ginseng (TSF) were investigated in this study. Firstly, a HPLC-ELSD chromatographic method was established to simultaneously determine the contents of 22 saponins in TSW and TSF. Secondly, CS-induced COPD mouse model was established to evaluate the activity of TSW and TSF. The results indicated that both TSW and TSF had the protective effect against COPD by alleviating oxidative stress and inflammatory response. TSW showed a stronger effect than TSF. Thirdly, an integrated approach involving metabolomics and network pharmacology was used to construct the "biomarker-reaction-enzyme-target" correlation network aiming at further exploring the observed effects. As the results, 15 biomarkers, 9 targets and 5 pathways were identified to play vital roles in the treatment of TSW and TSF on COPD. Fourthly, based on network pharmacology and the CS-stimulated A549 cell model, ginsenoside Rgl, Rc, oleanolic acid, notoginsenoside R1, Fe, silphioside B were certified to be the material basis for the stronger effect of TSW than TSF. Finally, the molecular docking were performed to visualize the binding modes. Our findings suggested that both TSW and TSF could effectively ameliorate the progression of COPD and might be used for the treatment of COPD.
(Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)