Quantitative BONCAT Allows Identification of Newly Synthesized Proteins after Optic Nerve Injury.

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Tytuł:: Quantitative BONCAT Allows Identification of Newly Synthesized Proteins after Optic Nerve Injury.
Autorzy:: Shah SH; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303.; Neuroscience Department and Dorris Neuroscience Center, Scripps Research, La Jolla, California 92093.; Neurosciences Graduate Program and Medical Scientist Training Program, University of California, San Diego, La Jolla, California 92093.
Schiapparelli LM; Neuroscience Department and Dorris Neuroscience Center, Scripps Research, La Jolla, California 92093.; Department of Cell Biology, Duke University Medical School, Durham, North Carolina, 27708.
Yokota S; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303.
Ma Y; Department of Molecular Medicine, Scripps Research, La Jolla, California 92093.
Xia X; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303.
Shankar S; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303.
Saturday S; Neuroscience Department and Dorris Neuroscience Center, Scripps Research, La Jolla, California 92093.
Nahmou M; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303.
Sun C; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303.
Yates J 3rd; Department of Molecular Medicine, Scripps Research, La Jolla, California 92093.
Cline HT; Neuroscience Department and Dorris Neuroscience Center, Scripps Research, La Jolla, California 92093.
Goldberg JL; Mary M. and Sash A. Spencer Center for Vision Research, Byers Eye Institute, Stanford University, Palo Alto, California 94303 .
Źródło:: The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2022 May 11; Vol. 42 (19), pp. 4042-4052. Date of Electronic Publication: 2022 Apr 08.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: Washington, DC : Society for Neuroscience
Original Publication: [Baltimore, Md.] : The Society, c1981-
MeSH Terms:: Optic Nerve Injuries*/metabolism
Axons/metabolism ; Humans ; Nerve Regeneration/physiology ; Retina/metabolism ; Retinal Ganglion Cells/metabolism
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Grant Information:: P41 GM103533 United States GM NIGMS NIH HHS; R01 MH067880 United States MH NIMH NIH HHS; P30 EY026877 United States EY NEI NIH HHS; R01 MH103134 United States MH NIMH NIH HHS; P30 EY019005 United States EY NEI NIH HHS; R01 EY027437 United States EY NEI NIH HHS; R01 EY011261 United States EY NEI NIH HHS; U01 EY027261 United States EY NEI NIH HHS
Contributed Indexing:: Keywords: neurodegeneration; optic nerve; protein synthesis; proteomics; retina
Entry Date(s):: Date Created: 20220409 Date Completed: 20220517 Latest Revision: 20240401
Update Code:: 20240402
PubMed Central ID:: PMC9097770
DOI:: 10.1523/JNEUROSCI.3100-20.2022
PMID:: 35396330
: Czasopismo naukowe

Retinal ganglion cells (RGCs) die after optic nerve trauma or in degenerative disease. However, acute changes in protein expression that may regulate RGC response to injury are not fully understood, and detailed methods to quantify new protein synthesis have not been tested. Here, we develop and apply a new in vivo quantitative measure of newly synthesized proteins to examine changes occurring in the retina after optic nerve injury. Azidohomoalanine, a noncanonical amino acid, was injected intravitreally into the eyes of rodents of either sex with or without optic nerve injury. Isotope variants of biotin-alkyne were used for quantitative BONCAT (QBONCAT) mass spectrometry, allowing identification of protein synthesis and transport rate changes in more than 1000 proteins at 1 or 5 d after optic nerve injury. In vitro screening showed several newly synthesized proteins regulate axon outgrowth in primary neurons in vitro This novel approach to targeted quantification of newly synthesized proteins after injury uncovers a dynamic translational response within broader proteostasis regulation and enhances our understanding of the cellular response to injury. SIGNIFICANCE STATEMENT Optic nerve injury results in death and degeneration of retinal ganglion cells and their axons. The specific cellular response to injury, including changes in new protein synthesis, is obscured by existing proteins and protein degradation. In this study, we introduce QBONCAT to isolate and quantify acute protein synthesis and subsequent transport between cellular compartments. We identify novel candidate protein effectors of the regenerative response and uncover their regulation of axon growth in vitro , validating the utility of QBONCAT for the discovery of novel regulatory and therapeutic candidates after optic nerve injury.
(Copyright © 2022 the authors.)

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