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Tytuł:
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A novel model of traumatic femoral head necrosis in rats developed by microsurgical technique.
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Autorzy:
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Lv Y; Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, 221000, Xuzhou, Jiangsu, China.
Qiu X; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, 116000, Dalian, Liaoning, China.
Liu G; Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, 221000, Xuzhou, Jiangsu, China.
Wang Y; Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, 221000, Xuzhou, Jiangsu, China.
Zhang Y; Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, 221000, Xuzhou, Jiangsu, China.
Li W; Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, 221000, Xuzhou, Jiangsu, China.
Zhu Z; Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, 221000, Xuzhou, Jiangsu, China. .
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Źródło:
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BMC musculoskeletal disorders [BMC Musculoskelet Disord] 2022 Apr 21; Vol. 23 (1), pp. 374. Date of Electronic Publication: 2022 Apr 21.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: London : BioMed Central, [2000-
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MeSH Terms:
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Femur Head Necrosis*/diagnostic imaging
Femur Head Necrosis*/etiology
Femur Head Necrosis*/surgery
Soft Tissue Injuries*/pathology
Animals ; Epiphyses/surgery ; Femur/pathology ; Femur Head/blood supply ; Femur Head/diagnostic imaging ; Femur Head/surgery ; Humans ; Rats ; X-Ray Microtomography
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References:
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Contributed Indexing:
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Keywords: Animal model; Femoral head traumatic osteonecrosis; Femoral neck fracture; Vasculature deprivation
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Entry Date(s):
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Date Created: 20220422 Date Completed: 20220425 Latest Revision: 20220716
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Update Code:
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20240104
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PubMed Central ID:
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PMC9022312
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DOI:
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10.1186/s12891-022-05289-7
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PMID:
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35449009
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Background: Clinical angiography and vascular microperfusion confirmed that the femoral head retains blood supply after a collum femur fracture. However, no animal model accurately mimics this clinical situation. This study was performed to establish a rat model with retained viability of the femoral head and partial vasculature deprivation-induced traumatic caput femoris necrosis by surgery.
Methods: Thirty rats were randomly divided into three groups (n = 10 per group): normal group, sham-operated group (Control), and ischemic osteonecrosis group. The femoral head of the normal group of rats underwent a gross anatomy study and microangiography to identify femoral head blood supply. Microsurgical techniques were used to cauterize the anterior-superior retinacular vessels to induce osteonecrosis. Hematoxylin and Eosin (H&E) staining were used for femoral head histologic assessment. Morphologic assessments of the deformity in and trabecular bone parameters of the femoral head epiphysis were performed using micro-CT.
Results: The blood supply of the femoral head in rats primarily came from the anterior-superior, inferior, and posterior retinacular arteries. However, anterior-superior retinacular vasculature deprivation alone was sufficient in inducing femoral head osteonecrosis. H&E showed bone cell loss in nuclear staining, disorganized marrow, and trabecular structure. The bone volume (BV) decreased by 13% and 22% in the ischemic group after 5 and 10 weeks, respectively. The mean trabecular thickness (Tb.Th) decreased from 0.09 to 0.06 mm after 10 weeks. The trabecular spacing (Tb.Sp) increased from 0.03 to 0.05 mm after 5 weeks, and the epiphyseal height-to-diameter (H/D) ratio decreased.
Conclusions: We developed an original and highly selective rat model that embodied femoral head traumatic osteonecrosis induced by surgical anterior-superior retinacular vasculature deprivation.
(© 2022. The Author(s).)
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