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Tytuł:
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Detection of muscle stem cell-derived myonuclei in murine overloaded muscles.
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Autorzy:
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Kaneshige A; Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.; Biological/Pharmacological Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan.; Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Kaji T; Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Saito H; Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Higashimoto T; Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Nakamura A; Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Kurosawa T; Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.; Laboratory of Veterinary Pharmacology, Department of Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences, Tokyo University, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Ikemoto-Uezumi M; Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
Uezumi A; Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
Fukada SI; Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
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Źródło:
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STAR protocols [STAR Protoc] 2022 Apr 11; Vol. 3 (2), pp. 101307. Date of Electronic Publication: 2022 Apr 11 (Print Publication: 2022).
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: [Cambridge, MA] : Cell Press, [2020]-
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MeSH Terms:
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Satellite Cells, Skeletal Muscle*
Animals ; Cell Nucleus ; Mice ; Muscle, Skeletal
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References:
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Cell Stem Cell. 2022 Feb 3;29(2):265-280.e6. (PMID: 34856120)
Nature. 2009 Jul 30;460(7255):627-31. (PMID: 19554048)
FASEB J. 2014 Apr;28(4):1654-65. (PMID: 24376025)
Skelet Muscle. 2017 Jul 10;7(1):14. (PMID: 28693603)
Elife. 2019 Sep 23;8:. (PMID: 31545169)
Biochim Biophys Acta Mol Cell Res. 2020 Sep;1867(9):118742. (PMID: 32417255)
Development. 2011 Sep;138(17):3657-66. (PMID: 21828094)
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Contributed Indexing:
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Keywords: Cell Biology; Microscopy; Stem Cells
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Entry Date(s):
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Date Created: 20220425 Date Completed: 20220426 Latest Revision: 20220629
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Update Code:
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20240104
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PubMed Central ID:
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PMC9018451
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DOI:
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10.1016/j.xpro.2022.101307
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PMID:
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35463471
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Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU + myonuclei and image acquisition for quantitative analyses. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022).
Competing Interests: The authors declare no competing financial interests.
(© 2022 The Author(s).)