-
Tytuł:
-
Assessing the effect of anesthetic gas mixtures on hyperpolarized C pyruvate metabolism in the rat brain.
-
Autorzy:
-
Healicon R; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.
Rooney CHE; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.
Ball V; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.
Shinozaki A; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.; Oxford Centre for Clinical Magnetic Resonance Research, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom.
Miller JJ; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.; Oxford Centre for Clinical Magnetic Resonance Research, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom.; Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom.; The PET Centre and The MR Centre, Clinical Medicine, Aarhus University and Aarhus University Hospital, Aarhus, Denmark.
Smart S; Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom.
Radford-Smith D; Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
Anthony D; Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
Tyler DJ; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.; The PET Centre and The MR Centre, Clinical Medicine, Aarhus University and Aarhus University Hospital, Aarhus, Denmark.
Grist JT; Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom.; The PET Centre and The MR Centre, Clinical Medicine, Aarhus University and Aarhus University Hospital, Aarhus, Denmark.; Department of Radiology, Oxford University Hospitals, Oxford, United Kingdom.; Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, United Kingdom.
-
Źródło:
-
Magnetic resonance in medicine [Magn Reson Med] 2022 Sep; Vol. 88 (3), pp. 1324-1332. Date of Electronic Publication: 2022 Apr 25.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: 1999- : New York, NY : Wiley
Original Publication: San Diego : Academic Press,
-
MeSH Terms:
-
Anesthetics, Inhalation*/pharmacology
Bicarbonates*/metabolism
Animals ; Brain/metabolism ; Carbon Isotopes/metabolism ; Magnetic Resonance Imaging/methods ; Oxygen/metabolism ; Pyruvic Acid/metabolism ; Rats
-
References:
-
J Magn Reson. 2019 Dec;309:106617. (PMID: 31648132)
Magn Reson Med. 2016 Apr;75(4):1515-24. (PMID: 25991606)
NMR Biomed. 2014 Oct;27(10):1256-65. (PMID: 25156807)
J Cereb Blood Flow Metab. 2020 Jun;40(6):1137-1147. (PMID: 32153235)
Int J Biomed Comput. 1983 Sep;14(5):411-29. (PMID: 6642751)
Magn Reson Med. 2018 Sep;80(3):864-873. (PMID: 29322616)
NMR Biomed. 2013 Oct;26(10):1197-203. (PMID: 23553852)
J Cereb Blood Flow Metab. 2007 Jan;27(1):69-75. (PMID: 16670698)
Magn Reson Med. 2013 Oct;70(4):1117-24. (PMID: 23086864)
J Cereb Blood Flow Metab. 2016 Jun;36(6):1046-58. (PMID: 26661163)
Magn Reson Med. 2022 Sep;88(3):1324-1332. (PMID: 35468245)
Neuroimage. 2009 May 1;45(4):1126-34. (PMID: 19118633)
Int J Mol Sci. 2017 Sep 07;18(9):. (PMID: 28880206)
Sci Rep. 2017 May 15;7(1):1907. (PMID: 28507314)
Neuroimage Clin. 2016 Jun 23;12:180-9. (PMID: 27437179)
Neuro Oncol. 2013 Apr;15(4):433-41. (PMID: 23328814)
Magn Reson Med. 2016 Apr;75(4):1474-83. (PMID: 25991580)
Magn Reson Med. 2010 May;63(5):1137-43. (PMID: 20432284)
J Cereb Blood Flow Metab. 2005 Oct;25(10):1394-9. (PMID: 15843788)
NMR Biomed. 2022 Feb;35(2):e4635. (PMID: 34672399)
NMR Biomed. 2016 Apr;29(4):387-99. (PMID: 27414749)
Br J Radiol. 2018 May;91(1085):20170688. (PMID: 29293376)
Sci Rep. 2017 Dec 13;7(1):17525. (PMID: 29235509)
Magn Reson Med. 2016 Mar;75(3):973-84. (PMID: 25946547)
Circ Res. 2002 Nov 29;91(11):1031-7. (PMID: 12456489)
Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10158-63. (PMID: 12930897)
Neuroimage. 2019 Apr 1;189:171-179. (PMID: 30639333)
Stroke. 1984 Jan-Feb;15(1):129-33. (PMID: 6695417)
J Appl Physiol (1985). 2003 Dec;95(6):2453-61. (PMID: 12937024)
Cancer Res. 2018 Jul 15;78(14):3755-3760. (PMID: 29769199)
Anesthesiology. 2019 Jun;130(6):1064-1077. (PMID: 30998510)
J Cereb Blood Flow Metab. 2005 Feb;25(2):204-16. (PMID: 15678123)
Circ Res. 2020 Mar 13;126(6):725-736. (PMID: 32078413)
Stroke. 1974 Sep-Oct;5(5):630-9. (PMID: 4472361)
J Magn Reson Imaging. 2011 Jun;33(6):1284-90. (PMID: 21590996)
Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):E6982-E6991. (PMID: 28760957)
Neuroimage. 2020 Jan 1;204:116202. (PMID: 31557546)
Sci Rep. 2018 Oct 10;8(1):15082. (PMID: 30305655)
NMR Biomed. 2021 Mar;34(3):e4460. (PMID: 33291188)
Br J Anaesth. 1990 Aug;65(2):210-5. (PMID: 2223338)
Circ Res. 2018 Apr 13;122(8):1084-1093. (PMID: 29440071)
-
Grant Information:
-
FS/19/18/34252 United Kingdom BHF_ British Heart Foundation; G0601490 United Kingdom MRC_ Medical Research Council; FS/14/17/30634 United Kingdom BHF_ British Heart Foundation; EP/L016052/1 United Kingdom MRC_ Medical Research Council; BRT00030 AV20 United Kingdom MRC_ Medical Research Council; FS/10/002/28078 United Kingdom BHF_ British Heart Foundation
-
Contributed Indexing:
-
Keywords: MRI; brain; hyperpolarized; metabolism
-
Substance Nomenclature:
-
0 (Anesthetics, Inhalation)
0 (Bicarbonates)
0 (Carbon Isotopes)
8558G7RUTR (Pyruvic Acid)
S88TT14065 (Oxygen)
-
Entry Date(s):
-
Date Created: 20220425 Date Completed: 20220630 Latest Revision: 20230322
-
Update Code:
-
20240104
-
PubMed Central ID:
-
PMC9325476
-
DOI:
-
10.1002/mrm.29274
-
PMID:
-
35468245
-
Purpose: To determine the effect of altering anesthetic oxygen protocols on measurements of cerebral perfusion and metabolism in the rodent brain.
Methods: Seven rats were anesthetized and underwent serial MRI scans with hyperpolarized [1- 13 C]pyruvate and perfusion weighted imaging. The anesthetic carrier gas protocol used varied from 100:0% to 90:10% to 60:40% O 2 :N 2 O. Spectra were quantified with AMARES and perfusion imaging was processed using model-free deconvolution. A 1-way ANOVA was used to compare results across groups, with pairwise t tests performed with correction for multiple comparisons. Spearman's correlation analysis was performed between O 2 % and MR measurements.
Results: There was a significant increase in bicarbonate:total 13 C carbon and bicarbonate: 13 C pyruvate when moving between 100:0 to 90:10 and 100:0 to 60:40 O 2 :N 2 O % (0.02 ± 0.01 vs. 0.019 ± 0.005 and 0.02 ± 0.01 vs. 0.05 ± 0.02, respectively) and (0.04 ± 0.01 vs. 0.03 ± 0.01 and 0.04 ± 0.01 vs. 0.08 ± 0.02, respectively). There was a significant difference in 13 C pyruvate time to peak when moving between 100:0 to 90:10 and 100:0 to 60:40 O 2 :N 2 O % (13 ± 2 vs. 10 ± 1 and 13 ± 2 vs. 7.5 ± 0.5 s, respectively) as well as significant differences in cerebral blood flow (CBF) between gas protocols. Significant correlations between bicarbonate: 13 C pyruvate and gas protocol (ρ = -0.47), mean transit time and gas protocol (ρ = 0.41) and 13 C pyruvate time-to-peak and cerebral blood flow (ρ = -0.54) were also observed.
Conclusions: These results demonstrate that the detection and quantification of cerebral metabolism and perfusion is dependent on the oxygen protocol used in the anesthetized rodent brain.
(© 2022 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.)