Characterization and functional interrogation of the SARS-CoV-2 RNA interactome.

Szczegóły
Abstrakt

Tytuł:: Characterization and functional interrogation of the SARS-CoV-2 RNA interactome.
Autorzy:: Labeau A; Université Paris Cité, INSERM U944 CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France.
Fery-Simonian L; Université Paris Cité, INSERM U944 CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France.
Lefevre-Utile A; Université Paris Cité, INSERM U976, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France.
Pourcelot M; Université Paris Cité, INSERM U944 CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France.
Bonnet-Madin L; Université Paris Cité, INSERM U944 CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France.
Soumelis V; Université Paris Cité, INSERM U976, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France.
Lotteau V; Centre International de Recherche en Infectiologie (CIRI), Univ Lyon, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, 69007 Lyon, France.
Vidalain PO; Centre International de Recherche en Infectiologie (CIRI), Univ Lyon, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, 69007 Lyon, France.
Amara A; Université Paris Cité, INSERM U944 CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France. Electronic address: .
Meertens L; Université Paris Cité, INSERM U944 CNRS 7212, Institut de Recherche Saint-Louis, Hôpital Saint-Louis, 75010 Paris, France. Electronic address: .
Źródło:: Cell reports [Cell Rep] 2022 Apr 26; Vol. 39 (4), pp. 110744.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
MeSH Terms:: COVID-19*/genetics
RNA, Viral*/genetics
Humans ; Pandemics ; SARS-CoV-2/genetics ; Virus Replication/genetics
References:: Cell. 2021 Apr 29;184(9):2372-2383.e9. (PMID: 33743213)
Mol Cell. 2019 Apr 4;74(1):196-211.e11. (PMID: 30799147)
Virol J. 2019 May 27;16(1):69. (PMID: 31133031)
Genome Biol. 2003;4(7):223. (PMID: 12844354)
Cell. 2021 Aug 5;184(16):4220-4236.e13. (PMID: 34242578)
Plant Methods. 2018 Jan 31;14:9. (PMID: 29422942)
J Virol. 2011 Sep;85(17):8460-6. (PMID: 21490091)
mBio. 2021 Oct 26;12(5):e0131621. (PMID: 34517762)
Cell Mol Life Sci. 2021 Apr;78(7):3565-3576. (PMID: 33449149)
EMBO J. 2012 Oct 17;31(20):4020-34. (PMID: 22960638)
Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507)
Cell. 2021 Apr 29;184(9):2394-2411.e16. (PMID: 33743211)
Nat Microbiol. 2019 Dec;4(12):2369-2382. (PMID: 31384002)
Cell. 2021 Apr 1;184(7):1865-1883.e20. (PMID: 33636127)
Cell Rep. 2017 Dec 26;21(13):3900-3913. (PMID: 29281836)
Bioinformatics. 2009 Apr 15;25(8):1091-3. (PMID: 19237447)
Nature. 2021 May;593(7857):142-146. (PMID: 33780970)
J Virol. 2010 Sep;84(17):8691-9. (PMID: 20573828)
Elife. 2020 Dec 03;9:. (PMID: 33269701)
Curr Top Microbiol Immunol. 2018;419:1-42. (PMID: 28643204)
Euro Surveill. 2020 Jan;25(3):. (PMID: 31992387)
N Engl J Med. 2020 Feb 20;382(8):727-733. (PMID: 31978945)
Cell Res. 2020 Mar;30(3):269-271. (PMID: 32020029)
Hum Genet. 2016 Aug;135(8):851-67. (PMID: 27215579)
Cell. 2021 Jan 7;184(1):106-119.e14. (PMID: 33333024)
Cell Host Microbe. 2021 Feb 10;29(2):267-280.e5. (PMID: 33357464)
Mol Cell. 2021 Jul 1;81(13):2851-2867.e7. (PMID: 34118193)
Cell. 2020 May 14;181(4):914-921.e10. (PMID: 32330414)
Genome Biol. 2009;10(10):242. (PMID: 19857271)
Nat Rev Microbiol. 2021 Mar;19(3):155-170. (PMID: 33116300)
Elife. 2019 Jan 11;8:. (PMID: 30632963)
Cell Host Microbe. 2020 Dec 9;28(6):853-866.e5. (PMID: 33245857)
Open Biol. 2018 Oct 24;8(10):. (PMID: 30355755)
J Proteomics. 2014 Apr 04;100:37-43. (PMID: 24513533)
Cell. 2002 Feb 22;108(4):523-31. (PMID: 11909523)
Cell. 2020 Nov 25;183(5):1325-1339.e21. (PMID: 33080218)
Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):151-6. (PMID: 17185417)
Cell Mol Immunol. 2020 Sep;17(9):998-1000. (PMID: 32728199)
Med (N Y). 2021 Jan 15;2(1):99-112.e7. (PMID: 32838362)
Nat Commun. 2018 Aug 3;9(1):3068. (PMID: 30076308)
J Virol. 2000 Sep;74(17):8127-34. (PMID: 10933723)
EMBO J. 2014 Dec 17;33(24):2887-9. (PMID: 25404664)
Cell. 2021 Jan 7;184(1):76-91.e13. (PMID: 33147444)
Cell. 2021 Jan 7;184(1):120-132.e14. (PMID: 33382968)
Biochem Biophys Res Commun. 2019 Feb 26;510(1):65-71. (PMID: 30661786)
PLoS Pathog. 2020 Dec 7;16(12):e1009129. (PMID: 33284835)
J Exp Med. 2021 Apr 5;218(4):. (PMID: 33533916)
Cell. 2021 Jan 7;184(1):92-105.e16. (PMID: 33147445)
Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658)
Adv Virus Res. 2016;96:59-126. (PMID: 27712628)
Nucleic Acids Res. 2020 Jan 10;48(1):116-129. (PMID: 31701124)
Antiviral Res. 2013 Nov;100(2):487-99. (PMID: 24035833)
J Virol. 2013 Jun;87(12):6625-34. (PMID: 23552423)
RNA Biol. 2020 Mar;17(3):366-380. (PMID: 31829086)
J Virol. 2006 Mar;80(6):2976-86. (PMID: 16501107)
Cell. 2021 Apr 29;184(9):2348-2361.e6. (PMID: 33730597)
Nature. 2019 Oct;574(7777):259-263. (PMID: 31554973)
Nat Struct Mol Biol. 2020 Oct;27(10):959-966. (PMID: 32908316)
Mol Cell. 2021 Jul 1;81(13):2838-2850.e6. (PMID: 33989516)
Cell Mol Life Sci. 2013 Aug;70(15):2657-75. (PMID: 23069990)
Nat Struct Mol Biol. 2016 Apr;23(4):278-285. (PMID: 26926436)
Nat Commun. 2019 Apr 3;10(1):1523. (PMID: 30944313)
Nature. 2022 Feb;602(7898):676-681. (PMID: 35016198)
PLoS Pathog. 2011 Nov;7(11):e1002397. (PMID: 22114566)
Lancet. 2020 Nov 14;396(10262):1595-1606. (PMID: 33065034)
Virology. 2011 Feb 5;410(1):38-47. (PMID: 21093005)
Nature. 2020 Jul;583(7816):459-468. (PMID: 32353859)
Cell. 2020 Apr 16;181(2):325-345.e28. (PMID: 32302571)
Nat Microbiol. 2021 Mar;6(3):339-353. (PMID: 33349665)
Front Microbiol. 2014 Jun 17;5:296. (PMID: 24987391)
J Transl Med. 2020 Aug 18;18(1):319. (PMID: 32811513)
Science. 2020 Dec 4;370(6521):. (PMID: 33060197)
Contributed Indexing:: Keywords: CP: Microbiology; SARS-CoV-2 RNA interactome; SARS-CoV-2 infection inhibitors; comprehensive identification of RNA binding proteins by mass spectrometry, ChIRP-MS; host RNA binding proteins; host-dependency factors; severe acute respiratory syndrome coronavirus 2; siRNA screen
Substance Nomenclature:: 0 (RNA, Viral)
Entry Date(s):: Date Created: 20220427 Date Completed: 20220429 Latest Revision: 20220913
Update Code:: 20240104
PubMed Central ID:: PMC9040432
DOI:: 10.1016/j.celrep.2022.110744
PMID:: 35477000
: Czasopismo naukowe

Full Text Finder

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutralizing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 biology. Using a comprehensive identification of RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors that interact with the SARS-CoV-2 genome during infection. By systematically knocking down their expression in human lung epithelial cells, we find that the majority of the identified RBPs are SARS-CoV-2 proviral factors. In particular, we show that HNRNPA2B1, ILF3, QKI, and SFPQ interact with the SARS-CoV-2 genome and promote viral RNA amplification. Our study provides valuable resources for future investigations into the mechanisms of SARS-CoV-2 replication and the identification of host-centered antiviral therapies.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Informacja