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Tytuł:
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A novel Fiji/ImageJ plugin for the rapid analysis of blebbing cells.
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Autorzy:
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Vosatka KW; Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, NY, United States of America.
Lavenus SB; Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, NY, United States of America.
Logue JS; Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, NY, United States of America.
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Źródło:
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PloS one [PLoS One] 2022 Apr 29; Vol. 17 (4), pp. e0267740. Date of Electronic Publication: 2022 Apr 29 (Print Publication: 2022).
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: San Francisco, CA : Public Library of Science
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MeSH Terms:
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Cell Movement*
Cell Membrane/*metabolism
Cell Tracking/*methods
Actin Cytoskeleton/metabolism ; Actins/metabolism ; Biological Phenomena
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References:
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Substance Nomenclature:
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0 (Actins)
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Entry Date(s):
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Date Created: 20220429 Date Completed: 20220503 Latest Revision: 20220624
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Update Code:
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20240105
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PubMed Central ID:
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PMC9053814
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DOI:
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10.1371/journal.pone.0267740
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PMID:
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35486637
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When confined, cells have recently been shown to undergo a phenotypic switch to what has been termed, fast amoeboid (leader bleb-based) migration. However, as this is a nascent area of research, few tools are available for the rapid analysis of cell behavior. Here, we demonstrate that a novel Fiji/ImageJ-based plugin, Analyze_Blebs, can be used to quickly obtain cell migration parameters and morphometrics from time lapse images. As validation, we show that Analyze_Blebs can detect significant differences in cell migration and morphometrics, such as the largest bleb size, upon introducing different live markers of F-actin, including F-tractin and LifeAct tagged with green and red fluorescent proteins. We also demonstrate, using flow cytometry, that live markers increase total levels of F-actin. Furthermore, that F-tractin increases cell stiffness, which was found to correlate with a decrease in migration, thus reaffirming the importance of cell mechanics as a determinant of Leader Bleb-Based Migration (LBBM).
Competing Interests: The authors have declared that no competing interests exist.
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