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Tytuł pozycji:

LncRNA PART1 inhibits glioma proliferation and migration via miR-374b/SALL1 axis.

Tytuł:
LncRNA PART1 inhibits glioma proliferation and migration via miR-374b/SALL1 axis.
Autorzy:
Deng YW; Department of Neurosurgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, Hunan Province, PR China. Electronic address: .
Shu YG; Department of Neurosurgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, Hunan Province, PR China.
Sun SL; Department of Neurosurgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, Hunan Province, PR China.
Źródło:
Neurochemistry international [Neurochem Int] 2022 Jul; Vol. 157, pp. 105347. Date of Electronic Publication: 2022 Apr 29.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Oxford [Elmsford, N. Y.] Pergamon Press.
MeSH Terms:
Glioma*/genetics
Glioma*/pathology
MicroRNAs*/genetics
MicroRNAs*/metabolism
RNA, Long Noncoding*/genetics
RNA, Long Noncoding*/metabolism
Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Humans
Contributed Indexing:
Keywords: Glioma; LncRNA PART1; SALL1; miR-374b
Substance Nomenclature:
0 (MicroRNAs)
0 (RNA, Long Noncoding)
Entry Date(s):
Date Created: 20220501 Date Completed: 20220603 Latest Revision: 20220623
Update Code:
20240105
DOI:
10.1016/j.neuint.2022.105347
PMID:
35490895
Czasopismo naukowe
Background: Abnormal expression of lncRNA is involved in a diversity of diseases and plays a vital role in targeted therapy. However, few studies have been conducted on lncRNA PART1 in glioma. We aimed to investigate the function and the potential regulatory mechanism of lncRNA PART1/miR-374b/SALL1 axis in glioma.
Methods: qRT-PCR and western blotting detected genes and proteins expression. Dual-luciferase reporter assay was performed to examine the binding relationship of lncRNA PART1, miR-374b, and SALL1. MTT assay and clone formation assay were performed to detect the cell viability and proliferation. Transwell assay detected glioma cell migration. In vivo tumor development experiments detected changes in tumor size, volume, and weight of the tumor after overexpression of lncRNA PART1. Immunohistochemistry was used to detect ki-67, E-cadherin, and N-cadherin expression.
Results: The expression of lncRNA PART1 and SALL1 were down-regulated and miR-374b was up-regulated in different glioma cell lines. Overexpression of lncRNA PART1 inhibited glioma cell proliferation, migration, and epithelial mesenchymal transition (EMT). LncRNA PART1 targeted miR-374b to promote SALL1 expression. The knockdown of miR-374b inhibited glioma cell proliferation and migration and EMT by SALL1. What's more, overexpression of miR-374b or knockdown of SALL1 reversed the inhibitory effect of lncRNA PART1 on the proliferation, migration, and EMT of glioma cells. Furthermore, overexpression of lncRNA PART1 inhibited glioma growth in vivo.
Conclusion: LncRNA PART1 inhibited glioma proliferation and migration via miR-374b/SALL1 axis. These results might provide new insights for comprehending the complex lncRNA-miRNA network in gliomas.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)

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