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Tytuł:
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Integrated enzyme with stimuli-responsive coordination polymer for personal glucose meter-based portable immunoassay.
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Autorzy:
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Gao L; Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China.
Li Y; Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China.
Huang ZZ; Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China. Electronic address: .
Tan H; Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China; College of Biological and Food Engineering, Huaihua University, Huaihua, 418000, China. Electronic address: .
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Źródło:
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Analytica chimica acta [Anal Chim Acta] 2022 May 15; Vol. 1207, pp. 339774. Date of Electronic Publication: 2022 Apr 01.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Amsterdam.
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MeSH Terms:
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Carcinoembryonic Antigen*
Stimuli Responsive Polymers*
Alkaline Phosphatase/analysis ; Glucose ; Immunoassay ; Polymers/chemistry ; Starch
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Contributed Indexing:
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Keywords: Carcinoembryonic antigen; Coordination polymer; Enzyme immobilization; Immunoassay; Personal glucose meter
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Substance Nomenclature:
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0 (Carcinoembryonic Antigen)
0 (Polymers)
0 (Stimuli Responsive Polymers)
9005-25-8 (Starch)
EC 3.1.3.1 (Alkaline Phosphatase)
IY9XDZ35W2 (Glucose)
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Entry Date(s):
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Date Created: 20220501 Date Completed: 20220503 Latest Revision: 20220503
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Update Code:
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20240105
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DOI:
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10.1016/j.aca.2022.339774
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PMID:
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35491036
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Coordination polymers (CPs) with tunable structures and properties have been extensively explored in a variety of fields. In this work, we demonstrated the potential of stimuli-responsive CPs as a host of integrating enzymes to construct a portable immunoassay. By employing terbium ion (Tb 3+ ) as a metal node and guanine monophosphate (GMP) as a bridge ligand, an alkaline phosphatase (ALP)-responsive Tb/GMP CPs was fabricated, which allows amyloglucosidase (GA) to be integrated to form GA@Tb/GMP composite. Owing to the size-selectivity of Tb/GMP CPs as a host, the loaded GA was physically isolated from its substrate starch. However, Tb/GMP CPs is highly sensitive to ALP, which can hydrolyze the phosphate group of GMP to destroy the structure of Tb/GMP CPs, leading to the release of GA from GA@Tb/GMP composite. The released GA can digest starch to produce glucoses and give a measured signal by personal glucose meter (PGM). This finding leads to a PGM-based portable immunoassay for the quantitative analysis of carcinoembryonic antigen (CEA), and satisfactory results with a detection limit of 0.28 ng/mL have been achieved. The successful determination of CEA in serum samples demonstrates its potential in practical application. We believe that this work can provide a remarkable insight for the rational design of stimuli-responsive CPs for a wide of applications.
(Copyright © 2022 Elsevier B.V. All rights reserved.)