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Tytuł:
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CD9 expression in porcine blood CD4 T cells delineates two subsets with phenotypic characteristics of central and effector memory cells.
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Autorzy:
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Álvarez B; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Revilla C; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Moreno S; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Jiménez-Marín Á; Grupo de Inmunogenómica y Patogénesis Molecular (IMIBIC), Departamento de Genética, Universidad de Córdoba, Campus Universitario de Rabanales, 14014, Córdoba, Spain.
Ramos E; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Martínez de la Riva P; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Poderoso T; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Garrido JJ; Grupo de Inmunogenómica y Patogénesis Molecular (IMIBIC), Departamento de Genética, Universidad de Córdoba, Campus Universitario de Rabanales, 14014, Córdoba, Spain.
Ezquerra Á; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain. Electronic address: .
Domínguez J; Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
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Źródło:
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Developmental and comparative immunology [Dev Comp Immunol] 2022 Aug; Vol. 133, pp. 104431. Date of Electronic Publication: 2022 May 05.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Tarrytown Ny : Elsevier Science
Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004.
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MeSH Terms:
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CD4-Positive T-Lymphocytes*
T-Lymphocyte Subsets*
Animals ; Cell Differentiation ; Immunologic Memory ; Leukocyte Common Antigens ; Phenotype ; Swine
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Contributed Indexing:
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Keywords: CCR7; CD4 T cells; CD9; IFN-γ; Memory; Swine
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Substance Nomenclature:
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EC 3.1.3.48 (Leukocyte Common Antigens)
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Entry Date(s):
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Date Created: 20220508 Date Completed: 20220603 Latest Revision: 20220809
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Update Code:
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20240105
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DOI:
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10.1016/j.dci.2022.104431
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PMID:
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35526640
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In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4 + T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4 + T cell development. CD4 + 2E3 + CD8α - CD9 - cells are the least differentiated population of naïve cells, whereas the CD4 + 2E3 - CD8α + CD9 + and CD4 + 2E3 - CD8α + CD9 - cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9 + phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4 + T cell heterogeneity in swine.
(Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
