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Tytuł:
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The comet assay for the evaluation of gut content genotoxicity: Use in human studies as an early biomarker of colon cancer risk.
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Autorzy:
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Ristori S; NEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Scavone F; Department of Experimental and Clinical Biomedical Sciences Mario Serio, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Bartolozzi C; NEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Giovannelli L; NEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy. Electronic address: .
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Źródło:
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Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2022 Jun; Vol. 878, pp. 503477. Date of Electronic Publication: 2022 Feb 24.
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Typ publikacji:
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Journal Article; Review
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier, 1997-
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MeSH Terms:
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Colonic Neoplasms*/genetics
Animals ; Biomarkers ; Comet Assay ; Humans ; Water
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Contributed Indexing:
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Keywords: CRC biomarker; ENDO III; Fecal water genotoxicity; Fpg; Nutrition
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Substance Nomenclature:
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0 (Biomarkers)
059QF0KO0R (Water)
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Entry Date(s):
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Date Created: 20220601 Date Completed: 20220603 Latest Revision: 20220603
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Update Code:
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20240105
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DOI:
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10.1016/j.mrgentox.2022.503477
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PMID:
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35649683
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This short narrative review describes the use of the comet assay to evaluate the formation of genotoxic compounds in the gut lumen in human studies. The fecal water genotoxicity assay is based on ability of the gut content to induce genotoxicity in a cellular model, employing the aqueous component of the feces (fecal water) as this is supposed to contain most of the reactive species and to convey them to the intestinal epithelium. This non-invasive and low-cost assay has been demonstrated to be associated with colon cancer risk in animal models, and although the final validation against human tumors is lacking, it is widely used as a colo-rectal cancer risk biomarker in human nutritional intervention studies. The contribution given to the field of nutrition and cancer by the FW genotoxicity assay is highlighted, particularly in conjunction with other risk biomarkers, to shed light on the complex relationship among diet, microbiota, individual subject characteristics and the formation of genotoxic compounds in the gut.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
