EED is required for mouse primordial germ cell differentiation in the embryonic gonad.

Szczegóły
Abstrakt

Tytuł:: EED is required for mouse primordial germ cell differentiation in the embryonic gonad.
Autorzy:: Lowe MG; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA.
Yen MR; Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
Hsu FM; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA.
Hosohama L; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Hu Z; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Chitiashvili T; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
Hunt TJ; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Gorgy I; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Bernard M; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
Wamaitha SE; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Chen PY; Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
Clark AT; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA. Electronic address: .
Źródło:: Developmental cell [Dev Cell] 2022 Jun 20; Vol. 57 (12), pp. 1482-1495.e5. Date of Electronic Publication: 2022 Jun 08.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Cambridge, Mass. : Cell Press, c2001-
MeSH Terms:: Germ Cells*/metabolism
Histones*/genetics
Histones*/metabolism
Polycomb Repressive Complex 2*/genetics
Polycomb Repressive Complex 2*/metabolism
Animals ; Cell Differentiation/genetics ; DNA Methylation ; Ectoderm/embryology ; Female ; Gonads/metabolism ; Male ; Mice
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Grant Information:: R01 HD058047 United States HD NICHD NIH HHS; T32 GM007185 United States GM NIGMS NIH HHS
Contributed Indexing:: Keywords: DNMT1; EED; H3K27me3; PRC2; embryo; meiosis; ovary development; primordial germ cells; testis development
Substance Nomenclature:: 0 (Eed protein, mouse)
0 (Histones)
EC 2.1.1.43 (Polycomb Repressive Complex 2)
Entry Date(s):: Date Created: 20220609 Date Completed: 20220623 Latest Revision: 20230621
Update Code:: 20240105
PubMed Central ID:: PMC9278986
DOI:: 10.1016/j.devcel.2022.05.012
PMID:: 35679863
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Development of primordial germ cells (PGCs) is required for reproduction. During PGC development in mammals, major epigenetic remodeling occurs, which is hypothesized to establish an epigenetic landscape for sex-specific germ cell differentiation and gametogenesis. In order to address the role of embryonic ectoderm development (EED) and histone 3 lysine 27 trimethylation (H3K27me3) in this process, we created an EED conditional knockout mouse and show that EED is essential for regulating the timing of sex-specific PGC differentiation in both ovaries and testes, as well as X chromosome dosage decompensation in testes. Integrating chromatin and whole genome bisulfite sequencing of epiblast and PGCs, we identified a poised repressive signature of H3K27me3/DNA methylation that we propose is established in the epiblast where EED and DNMT1 interact. Thus, EED joins DNMT1 in regulating the timing of sex-specific PGC differentiation during the critical window when the gonadal niche cells specialize into an ovary or testis.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)

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